Detailed information of TA system TA142460

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3879..4143
• Replicon: plasmid pTHNK130-1
• Accession: NZ_CP045742
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: GHR40_RS22125
• Protein ID: WP_001331364.1
• Coordinates: 3991..4143 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	3879..3941 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHR40_RS22115	1402..2472	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
GHR40_RS22120	2491..3699	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	3879..3941	-	63	NuclAT_0	-	Antitoxin
-	3879..3941	-	63	NuclAT_0	-	Antitoxin
-	3879..3941	-	63	NuclAT_0	-	Antitoxin
-	3879..3941	-	63	NuclAT_0	-	Antitoxin
GHR40_RS22125	3991..4143	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
GHR40_RS22130	4215..4466	-	252	WP_001291964.1	hypothetical protein	-
GHR40_RS23475	5125..5301	-	177	WP_001054897.1	hypothetical protein	-
GHR40_RS22135	5693..5902	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
GHR40_RS22140	5974..6636	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
GHR40_RS22145	6707..8875	-	2169	WP_000698354.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-14 / fosA3 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-IIa / aadA5	-	1..180783	180783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T142460 WP_001331364.1 NZ_CP045742:3991-4143 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T142460 NZ_CP060703:c5402758-5402480 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAACGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT142460 NZ_CP045742:c3941-3879 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin