142446

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2838085..2838587 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GHR40_RS13430 Protein ID WP_000767829.1
Coordinates 2838333..2838587 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GHR40_RS13425 Protein ID WP_001259255.1
Coordinates 2838085..2838336 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS13400 2833263..2834330 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GHR40_RS13405 2834330..2835400 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
GHR40_RS13410 2835397..2836701 - 1305 WP_000009594.1 histidinol dehydrogenase -
GHR40_RS13415 2836707..2837606 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GHR40_RS13420 2837752..2837802 - 51 WP_001364200.1 his operon leader peptide -
GHR40_RS13425 2838085..2838336 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GHR40_RS13430 2838333..2838587 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GHR40_RS13435 2838670..2839494 + 825 WP_000754737.1 SDR family oxidoreductase -
GHR40_RS13440 2839540..2840469 + 930 WP_000803351.1 LysR family transcriptional regulator -
GHR40_RS13445 2840684..2840746 + 63 WP_010723108.1 membrane protein YoeI -
GHR40_RS13450 2840736..2842094 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GHR40_RS13455 2842273..2843331 + 1059 WP_000492339.1 transport protein YeeE -
GHR40_RS13460 2843345..2843572 + 228 WP_046613325.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T142446 WP_000767829.1 NZ_CP045741:2838333-2838587 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T142446 NZ_CP060700:c1318287-1318066 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATATCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT142446 WP_001259255.1 NZ_CP045741:2838085-2838336 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT142446 NZ_CP060700:c1318587-1318300 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References