Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1699338..1700065 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag GHR40_RS08125 Protein ID WP_000550189.1
Coordinates 1699338..1699652 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GHR40_RS08130 Protein ID WP_000560266.1
Coordinates 1699649..1700065 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS08105 1695496..1696482 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
GHR40_RS08110 1696561..1697253 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
GHR40_RS08115 1697330..1697833 - 504 WP_001333820.1 M48 family metallopeptidase -
GHR40_RS08120 1697918..1699054 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
GHR40_RS08125 1699338..1699652 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
GHR40_RS08130 1699649..1700065 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GHR40_RS08135 1700110..1702128 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
GHR40_RS08140 1702554..1704905 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T142440 WP_000550189.1 NZ_CP045741:1699338-1699652 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T142440 NZ_CP060694:173668-173931 [Aliarcobacter cryaerophilus]
ATGATAGTTGGTTTTTCCACAAAAGCTTGGGAAGATTATAAATATTGGGTACAAAATGATAAGCAAATAGTAAAAAGAAT
AAATTTATTATTAGAAGATATAAAAAGAAATCCATATGATTCAAATGGTATTGGAAAACCTGAAAGATTAAAGGAAAATT
ATAGTGGATATTTTTCAAGAAGAATTACAAGTGAGCATAGATTGGTTTACAAAATAGTAGATGATTTGATAATTATTGCA
CAGTGCAGATTTCACTATTTATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT142440 WP_000560266.1 NZ_CP045741:1699649-1700065 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT142440 NZ_CP060694:173420-173671 [Aliarcobacter cryaerophilus]
ATGCAAGTAGTTCAATATTCACAAGCAAGAAATAATTTACGAGAGATAATAAATAAAGTATGTGATAACTTTGAAGAGTA
TATTATTAGTACAAAAGATAATAAGCAAGCAGTTTTAATATCTCAAGAAGAGTACAACTCTATGAAAGAGACTATGTATT
TATTATCATCAAAAGCTAATAGAGATAGATTAAATGAAGCTATAGATGAGATAGAAAATTTAAAATTTATAAAAAAAGAT
ATTAGCTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References