Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 38709..39403 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag GHR40_RS00255 Protein ID WP_001263489.1
Coordinates 38709..39107 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag GHR40_RS00260 Protein ID WP_000554758.1
Coordinates 39110..39403 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 34297..34377 - 81 NuclAT_12 - -
- 34297..34377 - 81 NuclAT_12 - -
- 34297..34377 - 81 NuclAT_12 - -
- 34297..34377 - 81 NuclAT_12 - -
GHR40_RS00230 34973..35431 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
GHR40_RS00235 35692..37149 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
GHR40_RS00240 37206..37727 - 522 Protein_46 peptide chain release factor H -
GHR40_RS00245 37723..37929 - 207 Protein_47 RtcB family protein -
GHR40_RS00250 38247..38699 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
GHR40_RS00255 38709..39107 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GHR40_RS00260 39110..39403 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GHR40_RS00265 39455..40510 - 1056 WP_001226164.1 DNA polymerase IV -
GHR40_RS00270 40581..41366 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GHR40_RS00275 41338..43050 + 1713 Protein_53 flagellar biosynthesis protein FlhA -
GHR40_RS00280 43274..43771 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T142430 WP_001263489.1 NZ_CP045741:c39107-38709 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T142430 NZ_CP060678:c4563490-4563122 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT142430 WP_000554758.1 NZ_CP045741:c39403-39110 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT142430 NZ_CP060678:c4563708-4563487 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References