Detailed information of TA system TA142428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6065952..6066547
• Replicon: chromosome
• Accession: NZ_CP045739
• Organism: Pseudomonas aeruginosa strain AG1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: PaeAG1_RS28805
• Protein ID: WP_003117425.1
• Coordinates: 6066269..6066547 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PaeAG1_RS28800
• Protein ID: WP_003099268.1
• Coordinates: 6065952..6066257 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PaeAG1_RS28765	6062595..6062870	-	276	WP_033896065.1	mercury resistance system periplasmic binding protein MerP	-
PaeAG1_RS28770	6062886..6063236	-	351	WP_001294660.1	mercuric transport protein MerT	-
PaeAG1_RS28775	6063308..6063763	+	456	WP_033896066.1	Hg(II)-responsive transcriptional regulator	-
PaeAG1_RS28780	6064125..6064901	-	777	WP_033896079.1	hypothetical protein	-
PaeAG1_RS28785	6064926..6065039	-	114	Protein_5692	RepA	-
PaeAG1_RS28790	6065078..6065290	-	213	WP_025297679.1	AlpA family phage regulatory protein	-
PaeAG1_RS28800	6065952..6066257	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
PaeAG1_RS28805	6066269..6066547	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PaeAG1_RS28810	6066872..6069100	+	2229	WP_003113525.1	TonB-dependent receptor	-
PaeAG1_RS28815	6069170..6069817	-	648	WP_003095021.1	carbonate dehydratase	-
PaeAG1_RS28820	6069879..6071117	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T142428 WP_003117425.1 NZ_CP045739:c6066547-6066269 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T142428 NZ_CP060678:c4048726-4048313 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT142428 WP_003099268.1 NZ_CP045739:c6066257-6065952 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT142428 NZ_CP060678:c4048973-4048707 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5