Detailed information of TA system TA142412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4460456..4461126
• Replicon: chromosome
• Accession: NZ_CP045726
• Organism: Citrobacter freundii strain zone4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: GHT30_RS21205
• Protein ID: WP_046671374.1
• Coordinates: 4460824..4461126 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GHT30_RS21200
• Protein ID: WP_046671375.1
• Coordinates: 4460456..4460797 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT30_RS21170	4455997..4456755	+	759	WP_003031816.1	phosphonate C-P lyase system protein PhnK	-
GHT30_RS21175	4456845..4457525	+	681	WP_003841104.1	phosphonate C-P lyase system protein PhnL	-
GHT30_RS21180	4457522..4458658	+	1137	WP_049003546.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
GHT30_RS21185	4458661..4459215	+	555	WP_003841102.1	ribose 1,5-bisphosphokinase	-
GHT30_RS21190	4459202..4459636	+	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
GHT30_RS21195	4459645..4460403	+	759	WP_086529671.1	phosphonate metabolism protein PhnP	-
GHT30_RS21200	4460456..4460797	-	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
GHT30_RS21205	4460824..4461126	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHT30_RS21210	4461282..4461611	-	330	WP_003031807.1	hypothetical protein	-
GHT30_RS21215	4461681..4463945	-	2265	WP_115454677.1	hybrid sensor histidine kinase/response regulator	-
GHT30_RS21220	4464060..4465583	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T142412 WP_046671374.1 NZ_CP045726:c4461126-4460824 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T142412 NZ_CP060676:3766896-3767294 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT142412 WP_046671375.1 NZ_CP045726:c4460797-4460456 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT142412 NZ_CP060676:3766669-3766896 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6