Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 330077..330743 Replicon chromosome
Accession NZ_CP045726
Organism Citrobacter freundii strain zone4

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag GHT30_RS01520 Protein ID WP_003847996.1
Coordinates 330426..330743 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag GHT30_RS01515 Protein ID WP_003837894.1
Coordinates 330077..330373 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT30_RS01500 327253..327726 - 474 WP_003023524.1 transcription elongation factor GreB -
GHT30_RS01505 327952..328671 + 720 WP_001157751.1 two-component system response regulator OmpR -
GHT30_RS01510 328668..330020 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
GHT30_RS01515 330077..330373 - 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
GHT30_RS01520 330426..330743 - 318 WP_003847996.1 hypothetical protein Toxin
GHT30_RS01525 330866..332488 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
GHT30_RS01530 332867..334585 + 1719 WP_046671282.1 DUF4153 domain-containing protein -
GHT30_RS01535 334695..335573 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T142398 WP_003847996.1 NZ_CP045726:c330743-330426 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T142398 NZ_CP060676:c693343-693188 [Salmonella enterica]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT142398 WP_003837894.1 NZ_CP045726:c330373-330077 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT142398 NZ_CP060676:693355-693413 [Salmonella enterica]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References