Detailed information of TA system TA142381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 908123..908738
• Replicon: chromosome
• Accession: NZ_CP045718
• Organism: Vibrio cholerae O395 substr. TCP2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: GG844_RS04510
• Protein ID: WP_001162670.1
• Coordinates: 908451..908738 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GG844_RS04505
• Protein ID: WP_001232701.1
• Coordinates: 908123..908440 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GG844_RS04460	903215..903670	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
GG844_RS04465	903800..904675	-	876	WP_000259044.1	hypothetical protein	-
GG844_RS04475	905122..905604	-	483	WP_001895003.1	hypothetical protein	-
GG844_RS04480	905661..906491	-	831	WP_000056616.1	abortive infection family protein	-
GG844_RS04485	906696..907436	-	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
GG844_RS04490	907660..907698	-	39	WP_106019119.1	hypothetical protein	-
GG844_RS04495	907714..907839	-	126	WP_001944767.1	DUF645 family protein	-
GG844_RS19685	908016..908126	-	111	WP_134814058.1	DUF3265 domain-containing protein	-
GG844_RS04505	908123..908440	-	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
GG844_RS04510	908451..908738	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GG844_RS04515	909005..909185	-	181	Protein_863	DUF645 family protein	-
GG844_RS04520	909610..909843	-	234	WP_032482776.1	DUF3709 domain-containing protein	-
GG844_RS04525	910246..910464	-	219	WP_001917086.1	DUF3709 domain-containing protein	-
GG844_RS04530	910774..911427	-	654	WP_000226874.1	hypothetical protein	-
GG844_RS04535	911484..911993	-	510	WP_000405986.1	GNAT family N-acetyltransferase	-
GG844_RS04540	912235..912477	+	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GG844_RS04545	912496..912813	+	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GG844_RS19690	913007..913102	-	96	WP_012706049.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	833007..914523	81516

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T142381 WP_001162670.1 NZ_CP045718:c908738-908451 [Vibrio cholerae O395]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T142381 NZ_CP060674:187975-188259 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT142381 WP_001232701.1 NZ_CP045718:c908440-908123 [Vibrio cholerae O395]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT142381 NZ_CP060674:187743-187985 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36