Detailed information of TA system TA142362

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61144..61556
• Replicon: plasmid pFHI_NMBU_11_02
• Accession: NZ_CP045714
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: GHT02_RS26765
• Protein ID: WP_000775238.1
• Coordinates: 61395..61556 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	61144..61349 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS26735	56821..57342	+	522	WP_077221344.1	single-stranded DNA-binding protein	-
GHT02_RS26740	57399..57632	+	234	WP_000005975.1	DUF905 domain-containing protein	-
GHT02_RS26745	57693..59657	+	1965	WP_077221343.1	ParB/RepB/Spo0J family partition protein	-
GHT02_RS26750	59726..60160	+	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
GHT02_RS26755	60157..60876	+	720	WP_077221342.1	plasmid SOS inhibition protein A	-
GHT02_RS26760	60873..61187	+	315	WP_000872086.1	hypothetical protein	-
-	61144..61349	+	206	NuclAT_0	-	Antitoxin
-	61144..61349	+	206	NuclAT_0	-	Antitoxin
-	61144..61349	+	206	NuclAT_0	-	Antitoxin
-	61144..61349	+	206	NuclAT_0	-	Antitoxin
GHT02_RS26765	61395..61556	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
GHT02_RS26770	61649..61846	+	198	Protein_88	hypothetical protein	-
GHT02_RS26775	62516..62818	+	303	WP_024213649.1	hypothetical protein	-
GHT02_RS26780	62974..63261	+	288	WP_000107543.1	hypothetical protein	-
GHT02_RS26785	63380..64201	+	822	WP_001562881.1	DUF945 domain-containing protein	-
GHT02_RS26790	64498..65100	-	603	WP_077221319.1	transglycosylase SLT domain-containing protein	-
GHT02_RS26795	65421..65804	+	384	WP_001151535.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..108688	108688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T142362 WP_000775238.1 NZ_CP045714:61395-61556 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T142362 NZ_CP060672:c384240-383929 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACACTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin

Nucleotide

• Length: 206 bp

>AT142362 NZ_CP045714:61144-61349 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTCGTGTGGCAGAAGGACAAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin