Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4731884..4732386 Replicon chromosome
Accession NZ_CP045712
Organism Escherichia coli strain FHI_NMBU_11

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GHT02_RS22815 Protein ID WP_000767829.1
Coordinates 4732132..4732386 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GHT02_RS22810 Protein ID WP_001259255.1
Coordinates 4731884..4732135 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT02_RS22785 4727061..4728128 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GHT02_RS22790 4728128..4729198 - 1071 WP_000108992.1 histidinol-phosphate transaminase -
GHT02_RS22795 4729195..4730499 - 1305 WP_001342580.1 histidinol dehydrogenase -
GHT02_RS22800 4730505..4731404 - 900 WP_000566782.1 ATP phosphoribosyltransferase -
GHT02_RS22805 4731550..4731600 - 51 WP_001364200.1 his operon leader peptide -
GHT02_RS22810 4731884..4732135 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GHT02_RS22815 4732132..4732386 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GHT02_RS22820 4732469..4733293 + 825 WP_000754752.1 SDR family oxidoreductase -
GHT02_RS22825 4733339..4734268 + 930 WP_000803351.1 LysR family transcriptional regulator -
GHT02_RS22830 4734483..4734545 + 63 WP_010723108.1 membrane protein YoeI -
GHT02_RS22835 4734535..4735893 + 1359 WP_086218987.1 putrescine/proton symporter PlaP -
GHT02_RS22840 4736072..4737130 + 1059 WP_000492346.1 transport protein YeeE -
GHT02_RS22845 4737144..4737371 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T142347 WP_000767829.1 NZ_CP045712:4732132-4732386 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T142347 NZ_CP060670:1484562-1484717 [Salmonella enterica]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT142347 WP_001259255.1 NZ_CP045712:4731884-4732135 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT142347 NZ_CP060670:c1484550-1484492 [Salmonella enterica]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References