Detailed information of TA system TA142342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3461631..3462430
• Replicon: chromosome
• Accession: NZ_CP045712
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: GHT02_RS16640
• Protein ID: WP_000347267.1
• Coordinates: 3461631..3462095 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: GHT02_RS16645
• Protein ID: WP_001302819.1
• Coordinates: 3462095..3462430 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS16610	3456632..3457066	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
GHT02_RS16615	3457084..3457962	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GHT02_RS16620	3457952..3458731	-	780	WP_086218896.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
GHT02_RS16625	3458742..3459215	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
GHT02_RS16630	3459238..3460518	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
GHT02_RS16635	3460767..3461576	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
GHT02_RS16640	3461631..3462095	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
GHT02_RS16645	3462095..3462430	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
GHT02_RS16650	3462579..3464150	-	1572	WP_001273769.1	galactarate dehydratase	-
GHT02_RS16655	3464525..3465859	+	1335	WP_000599648.1	galactarate/glucarate/glycerate transporter GarP	-
GHT02_RS16660	3465875..3466645	+	771	WP_001058220.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T142342 WP_000347267.1 NZ_CP045712:c3462095-3461631 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T142342 NZ_CP060669:17701-18006 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT142342 WP_001302819.1 NZ_CP045712:c3462430-3462095 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT142342 NZ_CP060669:17481-17699 [Salmonella enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4