Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1580956..1581497 Replicon chromosome
Accession NZ_CP045712
Organism Escherichia coli strain FHI_NMBU_11

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GHT02_RS07835 Protein ID WP_000615976.1
Coordinates 1581219..1581497 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GHT02_RS07830 Protein ID WP_000729704.1
Coordinates 1580956..1581216 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT02_RS07805 1576739..1577524 - 786 Protein_1534 putative lateral flagellar export/assembly protein LafU -
GHT02_RS07810 1577496..1579208 + 1713 Protein_1535 flagellar biosynthesis protein FlhA -
GHT02_RS07815 1579313..1579591 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GHT02_RS07820 1579584..1579940 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
GHT02_RS07825 1579997..1580770 - 774 WP_000543895.1 C40 family peptidase -
GHT02_RS07830 1580956..1581216 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GHT02_RS07835 1581219..1581497 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GHT02_RS07840 1581653..1582393 + 741 WP_001225679.1 murein L,D-transpeptidase -
GHT02_RS07845 1582364..1583131 - 768 WP_000333380.1 class II glutamine amidotransferase -
GHT02_RS07850 1583337..1583915 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1579584..1590106 10522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T142336 WP_000615976.1 NZ_CP045712:1581219-1581497 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T142336 NZ_CP060668:3756963-3757181 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT142336 WP_000729704.1 NZ_CP045712:1580956-1581216 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT142336 NZ_CP060668:3756561-3756935 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References