Detailed information of TA system TA142318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5443480..5444135
• Replicon: chromosome
• Accession: NZ_CP045701
• Organism: Pseudomonas brassicacearum strain S-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q0SC68
• Locus tag: GFU70_RS23500
• Protein ID: WP_058543614.1
• Coordinates: 5443791..5444135 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GFU70_RS23495
• Protein ID: WP_003205391.1
• Coordinates: 5443480..5443794 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFU70_RS23475	5439152..5440327	-	1176	WP_153388881.1	aspartate aminotransferase family protein	-
GFU70_RS23480	5440427..5441344	+	918	WP_064106950.1	LysR family transcriptional regulator	-
GFU70_RS23485	5441562..5442575	+	1014	WP_153388882.1	diguanylate cyclase	-
GFU70_RS23490	5442680..5443384	+	705	WP_116641364.1	hypothetical protein	-
GFU70_RS23495	5443480..5443794	-	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
GFU70_RS23500	5443791..5444135	-	345	WP_058543614.1	hypothetical protein	Toxin
GFU70_RS23505	5444276..5445163	-	888	WP_003178131.1	heme o synthase	-
GFU70_RS23510	5445175..5445510	-	336	WP_058543612.1	cytochrome o ubiquinol oxidase subunit IV	-
GFU70_RS23515	5445511..5446137	-	627	WP_003205395.1	cytochrome o ubiquinol oxidase subunit III	-
GFU70_RS23520	5446141..5448171	-	2031	WP_058543611.1	cytochrome o ubiquinol oxidase subunit I	-
GFU70_RS23525	5448175..5449122	-	948	WP_058543610.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13769.78 Da
• Isoelectric Point: 8.0039

>T142318 WP_058543614.1 NZ_CP045701:c5444135-5443791 [Pseudomonas brassicacearum]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTCEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T142318 NZ_CP060666:c3821829-3821338 [Salmonella enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT142318 WP_003205391.1 NZ_CP045701:c5443794-5443480 [Pseudomonas brassicacearum]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT142318 NZ_CP060666:c3822119-3821826 [Salmonella enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A5Q0SC68