Detailed information of TA system TA142316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2162032..2162636
• Replicon: chromosome
• Accession: NZ_CP045701
• Organism: Pseudomonas brassicacearum strain S-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GFU70_RS09475
• Protein ID: WP_114731147.1
• Coordinates: 2162322..2162636 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6N1CCA5
• Locus tag: GFU70_RS09470
• Protein ID: WP_058545613.1
• Coordinates: 2162032..2162319 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFU70_RS09440	2157232..2157366	-	135	Protein_1861	helix-turn-helix transcriptional regulator	-
GFU70_RS09445	2157476..2158384	-	909	WP_153387922.1	AraC family transcriptional regulator	-
GFU70_RS09450	2158571..2159620	+	1050	WP_116642813.1	NAD(P)-dependent alcohol dehydrogenase	-
GFU70_RS09455	2159749..2160966	+	1218	WP_153387923.1	MFS transporter	-
GFU70_RS09460	2161120..2161356	+	237	WP_175360461.1	hypothetical protein	-
GFU70_RS09465	2161403..2161891	-	489	WP_116642811.1	hypothetical protein	-
GFU70_RS09470	2162032..2162319	-	288	WP_058545613.1	helix-turn-helix domain-containing protein	Antitoxin
GFU70_RS09475	2162322..2162636	-	315	WP_114731147.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GFU70_RS09480	2162768..2163199	+	432	WP_153387924.1	GNAT family N-acetyltransferase	-
GFU70_RS09485	2163196..2164554	-	1359	WP_153387925.1	chromate efflux transporter	-
GFU70_RS09490	2164750..2165409	+	660	WP_058545617.1	ribonuclease T2	-
GFU70_RS09495	2165799..2167370	+	1572	WP_058545618.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11952.07 Da
• Isoelectric Point: 10.1359

>T142316 WP_114731147.1 NZ_CP045701:c2162636-2162322 [Pseudomonas brassicacearum]
MIFIETPIFTRRVKELMDDDDFAVLQKLLVCNPSAGDVMEATGGIRKIRIAAKGHGKRGGARVIYYHFVHASRIALLMIY
PKNEQQDLTVDQRKALKMIVEHWR

Nucleotide

• Length: 315 bp

>T142316 NZ_CP060666:c3673625-3673317 [Salmonella enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10741.40 Da
• Isoelectric Point: 8.7885

>AT142316 WP_058545613.1 NZ_CP045701:c2162319-2162032 [Pseudomonas brassicacearum]
MSKFFDELMESVQQMDEIVRGERQPSREFIVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVISALAV

Nucleotide

• Length: 288 bp

>AT142316 NZ_CP060666:c3673867-3673628 [Salmonella enterica]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6N1CCA5