Detailed information of TA system TA142309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 605722..606312
• Replicon: chromosome
• Accession: NZ_CP045698
• Organism: Brucella sp. 2280

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: GHC20_RS14260
• Protein ID: WP_153528321.1
• Coordinates: 606031..606312 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: GHC20_RS14255
• Protein ID: WP_153528320.1
• Coordinates: 605722..606018 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC20_RS14220	601270..601962	-	693	WP_153528316.1	DNA polymerase III subunit epsilon	-
GHC20_RS14225	602023..602220	-	198	WP_002969331.1	hypothetical protein	-
GHC20_RS14230	602367..602555	-	189	WP_008510705.1	hypothetical protein	-
GHC20_RS14235	602700..603005	-	306	WP_002971236.1	hypothetical protein	-
GHC20_RS14240	603146..603523	+	378	WP_153528317.1	pyrimidine dimer DNA glycosylase/endonuclease V	-
GHC20_RS14245	604428..605147	+	720	WP_153528318.1	GGDEF domain-containing protein	-
GHC20_RS14250	605277..605462	-	186	WP_153528319.1	hypothetical protein	-
GHC20_RS14255	605722..606018	-	297	WP_153528320.1	HigA family addiction module antidote protein	Antitoxin
GHC20_RS14260	606031..606312	-	282	WP_153528321.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHC20_RS14270	607029..609218	-	2190	WP_153528322.1	esterase-like activity of phytase family protein	-
GHC20_RS14275	609391..610731	-	1341	WP_153528323.1	DNA polymerase IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (14-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-79)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10510.92 Da
• Isoelectric Point: 9.0487

>T142309 WP_153528321.1 NZ_CP045698:c606312-606031 [Brucella sp. 2280]
MIRSYKNSATETVARGKAPKGFPSDLVRVSVRKLTMLDNAVTLDDLRSPPGNRLEALKGDRQGQHSIRINDQWRICFVWT
DAGAEQVEIVDYH

Nucleotide

• Length: 282 bp

>T142309 NZ_CP060666:477303-477701 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11015.78 Da
• Isoelectric Point: 5.6882

>AT142309 WP_153528320.1 NZ_CP045698:c606018-605722 [Brucella sp. 2280]
MTNILPPVHPGEILREEYLIPLGMSAGALAKRLNVPRTRIERLATEQTNVTPDTALRLAKFLRTTPEFWMNMQASYDLKT
EALAIQDQLAAIHEMEAA

Nucleotide

• Length: 297 bp

>AT142309 NZ_CP060666:477076-477303 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA