Detailed information of TA system TA142300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3883971..3884568
• Replicon: chromosome
• Accession: NZ_CP045694
• Organism: Klebsiella pneumoniae strain TK421

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: GHA50_RS20190
• Protein ID: WP_004893639.1
• Coordinates: 3884251..3884568 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: GHA50_RS20185
• Protein ID: WP_004142561.1
• Coordinates: 3883971..3884258 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHA50_RS20155	3880051..3880299	+	249	WP_032410665.1	DUF1158 domain-containing protein	-
GHA50_RS20160	3880317..3880658	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
GHA50_RS20165	3880689..3881804	-	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
GHA50_RS20170	3881984..3882565	+	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
GHA50_RS20175	3882565..3882933	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
GHA50_RS20180	3883053..3883706	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
GHA50_RS20185	3883971..3884258	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
GHA50_RS20190	3884251..3884568	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHA50_RS20195	3884753..3885796	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
GHA50_RS20200	3886462..3887328	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
GHA50_RS20205	3887437..3888804	+	1368	WP_153459393.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T142300 WP_004893639.1 NZ_CP045694:c3884568-3884251 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T142300 NZ_CP060641:1590618-1590953 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGTCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT142300 WP_004142561.1 NZ_CP045694:c3884258-3883971 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT142300 NZ_CP060641:1590341-1590628 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCTGTTACCTTTCAAGCTAATAAAGAATTGGTAAGTGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1