Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 29197..29924 Replicon plasmid unnamed1
Accession NZ_CP045662
Organism Klebsiella pneumoniae strain SMU18037509

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag GFJ44_RS25805 Protein ID WP_011251285.1
Coordinates 29613..29924 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GFJ44_RS25800 Protein ID WP_011251286.1
Coordinates 29197..29616 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFJ44_RS25780 24842..26047 - 1206 WP_048984478.1 restriction endonuclease subunit S -
GFJ44_RS25785 26037..27560 - 1524 WP_048984476.1 type I restriction-modification system subunit M -
GFJ44_RS25790 27829..28797 - 969 WP_194285226.1 IS5-like element IS903B family transposase -
GFJ44_RS25795 28819..29127 - 309 WP_053090837.1 hypothetical protein -
GFJ44_RS25800 29197..29616 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
GFJ44_RS25805 29613..29924 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
GFJ44_RS25810 30129..30587 - 459 Protein_31 DDE-type integrase/transposase/recombinase -
GFJ44_RS25815 30680..31018 + 339 WP_023288266.1 IS66 family insertion sequence hypothetical protein -
GFJ44_RS25820 31015..31362 + 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
GFJ44_RS25825 31411..32949 + 1539 WP_064188805.1 IS66 family transposase -
GFJ44_RS25830 32972..33340 + 369 Protein_35 transposase -
GFJ44_RS25835 33540..33974 - 435 WP_017900720.1 copper resistance system metallochaperone PcoE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..143263 143263
- inside IScluster/Tn - - 27829..32949 5120


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T142274 WP_011251285.1 NZ_CP045662:c29924-29613 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T142274 NZ_CP060584:699662-699766 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT142274 WP_011251286.1 NZ_CP045662:c29616-29197 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT142274 NZ_CP060584:c699925-699761 [Staphylococcus aureus]
GTAGCAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References