Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3764824..3765421 Replicon chromosome
Accession NZ_CP045641
Organism Klebsiella quasipneumoniae strain KP18-31

Toxin (Protein)


Gene name higB Uniprot ID A0A3T0QUS6
Locus tag GFK78_RS18165 Protein ID WP_017899012.1
Coordinates 3765104..3765421 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3T0QUV3
Locus tag GFK78_RS18160 Protein ID WP_017899013.1
Coordinates 3764824..3765111 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFK78_RS18130 3760911..3761159 + 249 WP_002893037.1 DUF1158 domain-containing protein -
GFK78_RS18135 3761177..3761518 - 342 WP_004202492.1 RamA family antibiotic efflux transcriptional regulator -
GFK78_RS18140 3761549..3762664 - 1116 WP_025999133.1 MBL fold metallo-hydrolase -
GFK78_RS18145 3762844..3763425 + 582 WP_017899014.1 TetR/AcrR family transcriptional regulator -
GFK78_RS18150 3763425..3763793 + 369 WP_032454994.1 MmcQ/YjbR family DNA-binding protein -
GFK78_RS18155 3763913..3764566 + 654 WP_004202485.1 oxygen-insensitive NAD(P)H nitroreductase -
GFK78_RS18160 3764824..3765111 - 288 WP_017899013.1 helix-turn-helix transcriptional regulator Antitoxin
GFK78_RS18165 3765104..3765421 - 318 WP_017899012.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GFK78_RS18170 3765606..3766649 - 1044 WP_060656299.1 hypothetical protein -
GFK78_RS18175 3767182..3768051 - 870 WP_135715832.1 helix-turn-helix transcriptional regulator -
GFK78_RS18180 3768160..3769587 + 1428 WP_135715833.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-87)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12185.52 Da        Isoelectric Point: 11.2767

>T142243 WP_017899012.1 NZ_CP045641:c3765421-3765104 [Klebsiella quasipneumoniae]
MFRMVVHVDVKKELQALPAIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQNKTLFLLRVF
VKKTQKTPLSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T142243 NZ_CP060491:731278-731382 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10840.52 Da        Isoelectric Point: 10.2213

>AT142243 WP_017899013.1 NZ_CP045641:c3765111-3764824 [Klebsiella quasipneumoniae]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQHRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRTSLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT142243 NZ_CP060491:c731541-731377 [Staphylococcus aureus]
GTAGCAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0QUS6

Antitoxin

Source ID Structure
AlphaFold DB A0A3T0QUV3

References