Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4702421..4703148 Replicon chromosome
Accession NZ_CP045611
Organism Enterobacter hormaechei strain AUH-ENM30

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag GBT00_RS22555 Protein ID WP_022650547.1
Coordinates 4702421..4702732 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag GBT00_RS22560 Protein ID WP_022650546.1
Coordinates 4702729..4703148 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBT00_RS22535 4698692..4699471 - 780 WP_003858848.1 acetolactate decarboxylase -
GBT00_RS22540 4699577..4700458 + 882 WP_022650550.1 LysR family transcriptional regulator -
GBT00_RS22545 4700741..4701196 + 456 WP_022650549.1 hypothetical protein -
GBT00_RS23455 4701309..4701728 - 420 WP_032645895.1 hypothetical protein -
GBT00_RS22555 4702421..4702732 + 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
GBT00_RS22560 4702729..4703148 + 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
GBT00_RS22565 4703472..4703906 + 435 WP_022650545.1 hypothetical protein -
GBT00_RS22570 4703959..4704849 - 891 WP_022650544.1 oxidoreductase -
GBT00_RS22575 4704955..4706076 + 1122 WP_003858868.1 glutamate--cysteine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T142200 WP_022650547.1 NZ_CP045611:4702421-4702732 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T142200 NZ_CP060446:5110854-5111270 [Xanthomonas citri pv. citri]
GTGATCCTGCTCGATACCAATGTCATCTCCGAACTTTGGCGGCCTCAGCCTAATCCGCAGGTCGTCGCCTGGATCGATGC
ACAAGCTGTCGAGACACTGTTCCTGTCGGTCGTGACGGTGGCTGAACTCCGTTTCGGTATTGCCGTGATGCCCAAGGGCC
GAAAGCGATCCACCTTGCATGCTCGGTTGGAGGGCGAGGTATTGCCACTGTTCGACGGGCGCCTGCTCGCATTCGATCTG
GATGCATCACACGCATTTGCGGCGCTTGCATCGAAAGCCAGAACCGCAGGACTCACACTCGGGCGCGCCGATGCCTACAT
CGCTGCAACAGCAGCCGCGCAAGGATTGACCGTTGCCACTCGCGACACGGCACCATTCGAGGCGATGGCGTTAGACGTCA
TTAACCCTTGGAGTTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT142200 WP_022650546.1 NZ_CP045611:4702729-4703148 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT142200 NZ_CP060446:5110603-5110857 [Xanthomonas citri pv. citri]
ATGCCTTCTTTCACCGTCCGCAATATCCCAGACGACGTGCATCGCGCGATCCGCGCGCGGGCTGCACTTCATGGCCGCAG
CACCGAAGCGGAGATCCGCGCAATCCTTGAATTGGCGGCCAAACCCGCAGACCGGGTCAAACTCGGCTCACTGCTGGTGA
GCATCGGCCGCGACGCCGGCCTGACCGCAAAAGAGGCCAATGCCTTCGACAAGCTGCGCGGCAAGGCGGCGATCAAGCCG
ATCGGTCTGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References