Detailed information of TA system TA142169

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2631565..2631864
• Replicon: chromosome
• Accession: NZ_CP045598
• Organism: Enterococcus faecalis strain TK-P4B

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: A0A8B3RWV6
• Locus tag: GFB65_RS12470
• Protein ID: WP_002424907.1
• Coordinates: 2631565..2631747 (+)
• Length: 61 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2631680..2631864 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB65_RS12450	2626873..2627088	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
GFB65_RS12455	2627227..2628219	+	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
GFB65_RS12460	2628483..2629136	-	654	WP_194848796.1	lytic polysaccharide monooxygenase	-
GFB65_RS12465	2629717..2631333	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-
GFB65_RS12470	2631565..2631747	+	183	WP_002424907.1	putative holin-like toxin	Toxin
-	2631680..2631864	-	185	NuclAT_7	-	Antitoxin
-	2631721..2631864	-	144	NuclAT_9	-	-
GFB65_RS12475	2631866..2632006	+	141	WP_073340360.1	putative holin-like toxin	-
-	2631939..2632140	-	202	NuclAT_6	-	-
GFB65_RS12480	2632234..2632377	+	144	WP_002396786.1	putative holin-like toxin	-
-	2632309..2632571	-	263	NuclAT_8	-	-
-	2632521..2632570	+	50	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ClpL	-	2536148..2640860	104712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (27-60)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6952.45 Da
• Isoelectric Point: 11.0738

>T142169 WP_002424907.1 NZ_CP045598:2631565-2631747 [Enterococcus faecalis]
MAVAIQSEREVMRMHVFPKFRERRGLLSAYETIQTILGFGMFTIALIALIVKLLKNDKKK

Nucleotide

• Length: 183 bp

>T142169 NZ_CP060440:c294113-293769 [Serratia marcescens]
ATGTGGCAAGTGGTGACCGTCGAACGGTTTGACGACTGGTTTTTAGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTACAAATTGCAGACGTTCGGCCCGCAATTGGCGCGGCCGCATGCCGATACGCTGCATTTTTCCGACGCGGTCC
GGCAGTTGAAGGAGCTGCGCATTCAACATCGCGGGCGCCCTTTCAGGGTGTTTTTCGCCTTTGATCCACAGCGGCAAGCG
GTATTGCTGTGCGGCGGCGATAAAACCGGCGACAAACGCTTTTACCAGCGCATGTTACCCATCGCCGCCATGGAGTTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Nucleotide

• Length: 185 bp

>AT142169 NZ_CP045598:c2631864-2631680 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A8B3RWV6

Antitoxin