Detailed information of TA system TA142163

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2453030..2453277
• Replicon: chromosome
• Accession: NZ_CP045598
• Organism: Enterococcus faecalis strain TK-P4B

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: -
• Locus tag: GFB65_RS11635
• Protein ID: WP_002386265.1
• Coordinates: 2453149..2453277 (-)
• Length: 43 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2453030..2453204 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB65_RS11610	2448677..2449630	-	954	WP_194848772.1	siderophore ABC transporter substrate-binding protein	-
GFB65_RS11615	2449669..2450424	-	756	WP_002354962.1	ATP-binding cassette domain-containing protein	-
GFB65_RS11620	2450421..2451386	-	966	WP_002367415.1	iron chelate uptake ABC transporter family permease subunit	-
GFB65_RS11625	2451383..2452330	-	948	WP_002354960.1	iron chelate uptake ABC transporter family permease subunit	-
GFB65_RS11630	2452515..2452967	+	453	WP_002378959.1	YueI family protein	-
-	2452993..2453200	+	208	NuclAT_1	-	-
-	2453030..2453204	+	175	NuclAT_5	-	Antitoxin
GFB65_RS11635	2453149..2453277	-	129	WP_002386265.1	hypothetical protein	Toxin
-	2453425..2453629	+	205	NuclAT_2	-	-
-	2453459..2453646	+	188	NuclAT_4	-	-
GFB65_RS11640	2453583..2453712	-	130	Protein_2265	putative holin-like toxin	-
GFB65_RS11645	2453875..2456145	-	2271	WP_048942005.1	bifunctional glutamate--cysteine ligase/glutathione synthetase	-
GFB65_RS11650	2456316..2456817	+	502	Protein_2267	cysteine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (11-42)

Sequences

Toxin

Protein

• Length: 43 a.a.
• Molecular weight: 4742.66 Da
• Isoelectric Point: 7.0058

>T142163 WP_002386265.1 NZ_CP045598:c2453277-2453149 [Enterococcus faecalis]
MHFNERSIFLSIEAALELMISFAAFVALLIFGILEATKNNKK

Nucleotide

• Length: 129 bp

>T142163 NZ_CP060421:c5342747-5342364 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCCTCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 175 bp

>AT142163 NZ_CP045598:2453030-2453204 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAAAA
TCAGTAGTGCAACAA

Structures

Antitoxin