Detailed information of TA system TA142152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 850959..851671
• Replicon: chromosome
• Accession: NZ_CP045572
• Organism: Nonomuraea nitratireducens strain WYY166

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: GBF35_RS04305
• Protein ID: WP_153261030.1
• Coordinates: 851285..851671 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GBF35_RS04300
• Protein ID: WP_139636610.1
• Coordinates: 850959..851288 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBF35_RS04290	846479..849289	+	2811	WP_153261028.1	hypothetical protein	-
GBF35_RS04295	849637..850518	+	882	WP_153261029.1	hypothetical protein	-
GBF35_RS04300	850959..851288	-	330	WP_139636610.1	helix-turn-helix transcriptional regulator	Antitoxin
GBF35_RS04305	851285..851671	-	387	WP_153261030.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBF35_RS04310	852174..853253	+	1080	WP_139636614.1	ABC transporter substrate-binding protein	-
GBF35_RS04315	853250..854083	+	834	WP_139636616.1	ABC transporter permease	-
GBF35_RS04320	854065..854967	+	903	WP_153261031.1	ABC transporter ATP-binding protein	-
GBF35_RS04325	855083..856606	+	1524	WP_139636620.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-96)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14564.67 Da
• Isoelectric Point: 4.8779

>T142152 WP_153261030.1 NZ_CP045572:c851671-851285 [Nonomuraea nitratireducens]
MAWEIVLLEPVESWFLKLCESDPDSAVLIERAIDRLAEVGPALGRPLVDTLIDGDMSNLKELRPGSRKRSEVRIVFVFDP
DREAIFLVAGDKAGRWSRWYEEAIPLAKARYTEYRAAKSAETKGEDKR

Nucleotide

• Length: 387 bp

>T142152 NZ_CP060417:c1322097-1321351 [Bacillus subtilis subsp. subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCCTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCGTCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11794.18 Da
• Isoelectric Point: 6.4889

>AT142152 WP_139636610.1 NZ_CP045572:c851288-850959 [Nonomuraea nitratireducens]
MSPGRNWREVKAEAHRLYPELADPQRQTDADAHLDAYVAGHHLKELRKSVGKTQSEVAAALGISQARVSQIENGDLDSME
LETIRAYAAALGGHVDVTISVGPHSVRVA

Nucleotide

• Length: 330 bp

>AT142152 NZ_CP060417:c1321351-1321181 [Bacillus subtilis subsp. subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA