Detailed information of TA system TA142134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 1290626..1291190
• Replicon: chromosome
• Accession: NZ_CP045567
• Organism: Lacticaseibacillus paracasei strain TK-P4A

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: GFB64_RS06370
• Protein ID: WP_194854101.1
• Coordinates: 1290888..1291190 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: K6SLT0
• Locus tag: GFB64_RS06365
• Protein ID: WP_003570051.1
• Coordinates: 1290626..1290895 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB64_RS06335	1285631..1286518	-	888	WP_016385537.1	ABC transporter ATP-binding protein	-
GFB64_RS06340	1286716..1287942	-	1227	WP_194854098.1	hypothetical protein	-
GFB64_RS06345	1288140..1288544	+	405	WP_045624944.1	MerR family transcriptional regulator	-
GFB64_RS06350	1288537..1288863	+	327	WP_194854099.1	YrdB family protein	-
GFB64_RS06355	1288925..1289554	+	630	Protein_1224	DUF2399 domain-containing protein	-
GFB64_RS06360	1289599..1290252	+	654	WP_194854100.1	GNAT family N-acetyltransferase	-
GFB64_RS06365	1290626..1290895	+	270	WP_003570051.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GFB64_RS06370	1290888..1291190	+	303	WP_194854101.1	type II toxin-antitoxin system YafQ family toxin	Toxin
GFB64_RS06375	1291529..1291672	+	144	WP_162259784.1	hypothetical protein	-
GFB64_RS06380	1291790..1292482	+	693	WP_194854102.1	hypothetical protein	-
GFB64_RS06385	1292707..1294884	+	2178	WP_194854103.1	RNA-binding transcriptional accessory protein	-
GFB64_RS06390	1294996..1295184	+	189	WP_194854104.1	hypothetical protein	-
GFB64_RS06395	1295186..1295566	+	381	Protein_1232	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-98)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11477.31 Da
• Isoelectric Point: 10.2819

>T142134 WP_194854101.1 NZ_CP045567:1290888-1291190 [Lacticaseibacillus paracasei]
MYNLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
HQDLILTLVRTGSHHNLLGK

Nucleotide

• Length: 303 bp

>T142134 NZ_CP060401:c3434009-3433509 [Xenorhabdus nematophila]
ATGATTCTTTCGGCACCGGAATTGCTGTCTGAACACCATTATCGTGAAGCGTTTAATTCAGGGGTTACCACCCTAGACCA
GTGGCTCAAAAATCGTGCACTAAAAAATAACTTAACTGGCGCATCAAAAACCTACGTGGTTTGTAGTGAAAATCGAGTAC
TGGCTTATTATTCTCTGGCCGCTAGCGCGATTGTGACTGATTCGACACCCGGCCATTTTAGGCGGAATATGCCTAACCCA
ATCCCTGTCGTCGTTTTAGGCCGATTGGCTGTAGATAGGAGTCTGCAACGAAAAGGCATAGGCCGTGCGTTAGTACAAGA
TGCCGCGTTGAGAATAATCCAAGCTGCTGATTTAATTGGTGTCCGAGGTATGATTATTCATGCGCTTTCAGATGAAGTAA
AATCTTTTTATGAAACCATCGGGTTTGAACCTTCTCCTCTCGATCCCATGTTACTGATGGCTACACTCGCAGATTTAAAA
AGATCCTGTACGAATAGTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9982.68 Da
• Isoelectric Point: 7.2026

>AT142134 WP_003570051.1 NZ_CP045567:1290626-1290895 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Nucleotide

• Length: 270 bp

>AT142134 NZ_CP060401:c3434284-3434006 [Xenorhabdus nematophila]
ATGCAAACGCCAAAGCGAGATACTTTGAGTATTCGTATCAAACCAGAAACTCGGAATTTGATTGATAAAGCAGCCGCCAT
TCAGGGTAAAAACCGCACAGATTTTGTATTGGAAGCGGCACAACGCATGGCCGAAGAAACCTTGCTTGAACAAGCAATTA
TGACGGCCAGTCCAGAAGCATATGCCAAATTTTTGGATCGTTTAGATATGCCTCCACAACCGAATAAGCAATTGCGAGAA
ACCATGCAGATGGAAACGCCTTGGGGCAAGGAATTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2BRK6