Detailed information of TA system TA142132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 164230..164815
• Replicon: plasmid pAC1530
• Accession: NZ_CP045561
• Organism: Acinetobacter nosocomialis strain AC1530

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: GD578_RS20020
• Protein ID: WP_000897307.1
• Coordinates: 164230..164550 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GD578_RS20025
• Protein ID: WP_000369781.1
• Coordinates: 164543..164815 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GD578_RS19980	159934..160761	+	828	WP_000073658.1	AraC family transcriptional regulator	-
GD578_RS19985	160811..161416	+	606	WP_000447788.1	LysE family translocator	-
GD578_RS19990	161466..161780	-	315	WP_004728120.1	BrnA antitoxin family protein	-
GD578_RS19995	161767..162054	-	288	WP_000438825.1	BrnT family toxin	-
GD578_RS20000	162326..162856	-	531	WP_000172759.1	hypothetical protein	-
GD578_RS20005	163005..163307	+	303	Protein_159	type II toxin-antitoxin system RelE/ParE family toxin	-
GD578_RS20010	163308..163589	+	282	WP_000985609.1	putative addiction module antidote protein	-
GD578_RS20015	163669..164088	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
GD578_RS20020	164230..164550	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GD578_RS20025	164543..164815	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
GD578_RS20030	165308..166783	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
GD578_RS20035	166839..167723	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
GD578_RS20040	167913..168524	-	612	WP_015060246.1	recombinase family protein	-
GD578_RS20045	168780..169742	+	963	WP_033917576.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / blaNDM-1 / aph(3'')-Ib / aph(6)-Id / blaOXA-58 / msr(E) / mph(E)	-	1..173972	173972
	-	inside	IScluster/Tn	aph(3'')-Ib / aph(6)-Id / blaOXA-58	-	147542..159491	11949
	-	flank	IS/Tn	msr(E) / mph(E)	-	165308..169742	4434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T142132 WP_000897307.1 NZ_CP045561:164230-164550 [Acinetobacter nosocomialis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T142132 NZ_CP060401:c3343394-3343089 [Xenorhabdus nematophila]
ATGCAGTACACAGTTTACAAAAATACAGGTAATCCTGATTATTCCTATCTGTTGGATGTTCAGAGCGACATTATTGATGT
ATTAGAAACACGATTGGTCATTCCATTATTCCTAAAATCAAATTTCCACGGGCGAATTCCAACTCGATTATGCCCGACCC
TGAACATTGATGGGAACGAATATCTCGTTATGACTCACGCGATGGCTAGCATCAGAGTCTCTATGTTAGGTGATGAGATG
ACAAATGTTTCATCCCACAGACAGCTAATTAAAGATGCGATGGATTTACTCTTTGACGGGTTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT142132 WP_000369781.1 NZ_CP045561:164543-164815 [Acinetobacter nosocomialis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT142132 NZ_CP060401:c3343635-3343396 [Xenorhabdus nematophila]
ATGACAGCATTACACTCCAGAACTAAAAAAACTGTCAGCGTGACTGTTTCACCAGAACTGTATGAACAAGCAAAACAAAT
AGGACTAAATTTTTCTGCTATTTTGACTCAGGCATTAATTGCAGAACTTAAATCTGCGGCGGCTGAGCAGTGGAAACGTG
ATAATAGGGAGGGGTTGGAAGAACTCAATCGCGTCACCCGTGAACATGGTCTACTGTCCGATCAATATAGGACATTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3