Detailed information of TA system TA142122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5800687..5801282
• Replicon: chromosome
• Accession: NZ_CP045552
• Organism: Pseudomonas aeruginosa strain YT12746

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: GEV42_RS27570
• Protein ID: WP_003117425.1
• Coordinates: 5801004..5801282 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GEV42_RS27565
• Protein ID: WP_003113527.1
• Coordinates: 5800687..5800992 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEV42_RS27540	5795800..5796090	-	291	WP_033992920.1	DUF5447 family protein	-
GEV42_RS27545	5796302..5796574	-	273	WP_003115921.1	hypothetical protein	-
GEV42_RS27550	5797104..5798201	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
GEV42_RS27555	5798204..5799253	+	1050	WP_003113529.1	serine/threonine protein kinase	-
GEV42_RS27560	5799250..5800323	+	1074	WP_173834565.1	serine/threonine protein kinase	-
GEV42_RS27565	5800687..5800992	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
GEV42_RS27570	5801004..5801282	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GEV42_RS27575	5801611..5803839	+	2229	WP_003099265.1	TonB-dependent receptor	-
GEV42_RS27580	5803909..5804556	-	648	WP_003095021.1	carbonate dehydratase	-
GEV42_RS27585	5804618..5805856	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T142122 WP_003117425.1 NZ_CP045552:c5801282-5801004 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T142122 NZ_CP060401:c1620695-1620207 [Xenorhabdus nematophila]
GTGGGAATAAGTACGCCGGAAATCTTAACGGTTAAACATAATGTAAAGGATTTTTATTGCCAGCATGAAACATTAAACGA
ATGGCTTTCACGCCGGGCTTTAAAAAATAATAAGCTCGGCGCGAGCCGCACATTTGTTATTTGCAAGGAAGGAACAAAAG
ACGTTATCGGTTATTATTGTCTAAGTGCTGGTTCAATTAATCATATTGAATCTACACCATCCCTTAAACGCAATATGCCA
GATGCTATCCCTGTAATGTTGCTCGGCCGCCTAGCTGTTGATGCGGAATATCAGGGGAAGAAGCTCGGTGCATTACTTCT
TCAAGATGCTTGGAAGCGTGTTGCCAGTACGGCGGAACAGGTAGGCATTTCAGCGATAATTGTCCACGCATTTGATGAAA
GCGCCCGAACGTTCTATATGCGTCTAGGTTTCGTCCAGTCACCATTGGAGTCATATACACTGATGCTTCCATTAGGCAAA
CAGAACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT142122 WP_003113527.1 NZ_CP045552:c5800992-5800687 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT142122 NZ_CP060401:c1620958-1620686 [Xenorhabdus nematophila]
ATGAGAACACAACCTAAAGAAACCCCGATTAACATCCGGGCGAAAGCCTTCCAGCGGGAACTTATCGACCATGCAGCAAA
CCTACATTCGAAGACACGAACTGATTTCATCTTGGATGCTGCCTGCCGAGCTGCTGAAGAGGCCATACTCGATCAGCGCC
ACTTTTTTGTTAACGACGAAAAATATCAGGCGTTCATGCAGATGCTGGAACAACCGCTTTCTGATAACGCAGGTTTTAAA
AAGCTCATGGGATATAAAGCACCGTGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5