Detailed information of TA system TA142100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1258124..1258694
• Replicon: chromosome
• Accession: NZ_CP045529
• Organism: Luteimicrobium xylanilyticum strain HY-24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5P9Q822
• Locus tag: KDY119_RS05375
• Protein ID: WP_036952070.1
• Coordinates: 1258124..1258405 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KDY119_RS05380
• Protein ID: WP_036952067.1
• Coordinates: 1258392..1258694 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDY119_RS05350	1253385..1254434	+	1050	WP_036952075.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
KDY119_RS05355	1254625..1254810	+	186	WP_148284216.1	hypothetical protein	-
KDY119_RS05360	1254850..1256349	-	1500	WP_051136705.1	DNA recombination protein RmuC	-
KDY119_RS05365	1256399..1256941	-	543	WP_036952902.1	GNAT family N-acetyltransferase	-
KDY119_RS05370	1257005..1258090	+	1086	WP_036952073.1	redox-regulated ATPase YchF	-
KDY119_RS05375	1258124..1258405	+	282	WP_036952070.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KDY119_RS05380	1258392..1258694	+	303	WP_036952067.1	HigA family addiction module antidote protein	Antitoxin
KDY119_RS05385	1258691..1259212	+	522	WP_036952065.1	ATP-binding protein	-
KDY119_RS18830	1259357..1259509	-	153	WP_194174348.1	AbiV family abortive infection protein	-
KDY119_RS05395	1259870..1260211	-	342	WP_036952062.1	hypothetical protein	-
KDY119_RS05400	1260328..1260858	+	531	WP_051136703.1	helix-turn-helix transcriptional regulator	-
KDY119_RS05405	1260909..1261378	+	470	Protein_1066	GNAT family N-acetyltransferase	-
KDY119_RS05410	1261439..1261849	-	411	WP_036952060.1	NAD(+)--rifampin ADP-ribosyltransferase	-
KDY119_RS05415	1261917..1262135	+	219	WP_036952058.1	hypothetical protein	-
KDY119_RS05420	1262132..1263067	+	936	WP_153022036.1	cation transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-97)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10972.53 Da
• Isoelectric Point: 10.9267

>T142100 WP_036952070.1 NZ_CP045529:1258124-1258405 [Luteimicrobium xylanilyticum]
MIRSFGDKATERLWRRERVRSIDPRIHRVALRKLRQVGSAETLEDLRVPPGNRLEALKGDRAGRHSIRINDQWRICFVWT
DAGPEEVQIVDYH

Nucleotide

• Length: 282 bp

>T142100 NZ_CP060397:c2580129-2579734 [Synechococcus sp. CBW1004]
CTGGTCGTGGACACCTCGGCCTTGATGGCGGTGCTGCTGCAAGAGAGCGACGCCGAGGTCCTTCTGGACGCGGCCGTGCG
GGCTTCAGCCCTGTACCTGAGCACCGCCACCCGGCTGGAGCTGAGCCTGGTGGCTGAAAGCGTGCGTCACGCCGTCAAAC
CCAGTGAGATCGAGCAGCTTCTGCTGAGCCTCCGGGTGGAACTGGTGCCCCTCGACAAGGAGCAGATGCACTGGGCCCTG
CAGGGCTGGCGCATCTACGGCAAGGGCCGACACGCGGCGAGCCTCAACCTGGGCGACTGCTTCTGCTACGGGCTGGCGAA
GGCCCTGCAGGTGCCGCTGCTGTTCAAGGGCGAGGACTTCGCCGCCACGGATCTGGAGCCGGCGCTGGCCCCCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11061.59 Da
• Isoelectric Point: 5.1100

>AT142100 WP_036952067.1 NZ_CP045529:1258392-1258694 [Luteimicrobium xylanilyticum]
MTTTDRIEPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRGITADTALRLAKFFGTSAEFWINLQSHYEL
DRAEDRAGEQIASITPLAVA

Nucleotide

• Length: 303 bp

>AT142100 NZ_CP060397:c2580416-2580147 [Synechococcus sp. CBW1004]
ATGGGCATGAACATCAAGGATCCCGAGGTGCACGCCATGGCCCGCGAGCTGGCGGCGCGGCGCTCCACCACCGTCACCGA
CGCGGTGCGGCAGGCCCTGCGGGCCGAACTCGAGCGCTGCCCCAGCACGGAACCGCCCTCCGCCATCGAGGCCAGAAAGG
CGGCGATTCGCGCCATCTGCGCCCGCGTCAGCGCCAGGAACGAATGGCAGGGTCTCTCCAGCAAGGAGCTGCAGGACGCC
CTCTATGACGAGGACGGGCTGCCAAAATGA

Structures

Toxin

• AlphaFold DB: A0A5P9Q822