Detailed information of TA system TA142086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1906314..1907041
• Replicon: chromosome
• Accession: NZ_CP045526
• Organism: Shigella sonnei strain 6607.69

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: GE169_RS10080
• Protein ID: WP_000547563.1
• Coordinates: 1906730..1907041 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GE169_RS10075
• Protein ID: WP_000126296.1
• Coordinates: 1906314..1906733 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE169_RS10055 (1901365)	1901365..1901892	-	528	WP_001078766.1	electron transport protein HydN	-
GE169_RS10060 (1902040)	1902040..1903050	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
GE169_RS10065 (1903310)	1903310..1904767	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GE169_RS10070 (1904776)	1904776..1906200	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
GE169_RS10075 (1906314)	1906314..1906733	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
GE169_RS10080 (1906730)	1906730..1907041	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
GE169_RS10085 (1907203)	1907203..1907976	-	774	WP_001026444.1	hypothetical protein	-
GE169_RS10090 (1908001)	1908001..1908471	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
GE169_RS10095 (1908464)	1908464..1908874	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
GE169_RS10100 (1908871)	1908871..1909638	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
GE169_RS10105 (1909638)	1909638..1910180	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
GE169_RS10110 (1910190)	1910190..1911887	-	1698	WP_112384455.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T142086 WP_000547563.1 NZ_CP045526:c1907041-1906730 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T142086 NZ_CP060396:c1812270-1811911 [Synechococcus sp. CBW1006]
TTGACTGCTGCGTATGTCCCTGATCGGGGTGACTTGGTCTGGCTGGAGTTCACACCGCAGACGGGTAGTGAGCAGGCTGG
GCGGCGTCCTGCTTTGGTGATCTCGCCAAAGGCCTACAACGCGAAGGTGGGCTTGGCCTTGTTTTGTCCAGTGACATCAC
GAGTCAAGGGTTATCCGTTTGAAGTGGCATTGCCTGAGGGCAGTGGTGTGGTCGGTGTCGTCCTTGCAGATCAGTTGAAG
AGCCTTGACTGGCGTGCCAGGAAGGCGAAGCTGATTGAGCGTGCTTCGTCTGATGTGCTGGCGATGGTTACAGCAAGGAT
CCTGCCCCTGCTTGTTCCTGACAATCCTGCCACTCTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT142086 WP_000126296.1 NZ_CP045526:c1906733-1906314 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT142086 NZ_CP060396:c1812512-1812267 [Synechococcus sp. CBW1006]
GTGCAGACGCGCGTCCAGAAATGGGGAAACAGTCTTGGGGTGCGGATCCCGCGCGGCCTGGCTGAGGAGGTTGGCCTCGG
CGCTGGCTCTGAAGTTAGCTTGAGTGCCAAGGATGGCGAGCTTGTCGTGAAGCCGTCGGTTCCTGCTCGCCTCAGCCTCG
ACGATCTTTTGGCAGAGGTCTCAGAGGAGAACCTCCATTCCTCTATCGATACGGGCTCTGCTGTCGGTGCGGAGATCTTC
TGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22