Detailed information of TA system TA142073

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27552..27978
• Replicon: plasmid p6904-27
• Accession: NZ_CP045525
• Organism: Shigella sonnei strain 6904.27

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: GE168_RS24300
• Protein ID: WP_001372321.1
• Coordinates: 27552..27677 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27754..27978 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE168_RS24265 (22575)	22575..22802	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
GE168_RS24270 (22939)	22939..23610	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
GE168_RS24275 (23804)	23804..24187	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
GE168_RS24280 (24522)	24522..25112	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
GE168_RS24285 (25409)	25409..26230	-	822	WP_001234445.1	DUF932 domain-containing protein	-
GE168_RS24290 (26341)	26341..26637	-	297	WP_001272251.1	hypothetical protein	-
GE168_RS24295 (26937)	26937..27233	+	297	Protein_36	hypothetical protein	-
GE168_RS24300 (27552)	27552..27677	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
GE168_RS24305 (27619)	27619..27768	-	150	Protein_38	plasmid maintenance protein Mok	-
GE168_RS24310 (27790)	27790..27969	+	180	WP_001309233.1	hypothetical protein	-
- (27754)	27754..27978	-	225	NuclAT_0	-	Antitoxin
- (27754)	27754..27978	-	225	NuclAT_0	-	Antitoxin
- (27754)	27754..27978	-	225	NuclAT_0	-	Antitoxin
- (27754)	27754..27978	-	225	NuclAT_0	-	Antitoxin
GE168_RS24315 (27990)	27990..28709	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
GE168_RS24320 (28706)	28706..29140	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GE168_RS24325 (29209)	29209..31232	-	2024	Protein_42	ParB/RepB/Spo0J family partition protein	-
GE168_RS24330 (31293)	31293..31526	-	234	WP_000006003.1	DUF905 family protein	-
GE168_RS24335 (31584)	31584..32111	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
GE168_RS24340 (32413)	32413..32862	+	450	Protein_45	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T142073 WP_001372321.1 NZ_CP045525:c27677-27552 [Shigella sonnei]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T142073 NZ_CP060395:1550434-1550835 [Synechococcus sp. CBW1108]
ATGGAACTCGTGATCGACCCCTCGGCTCTGATGGCCCTGCTGCTGCAGGAGGCAGAGGCAGAATCGCTGCTTGAAGTGGC
GGCTGAGGCTTCGGTTGTGCATCTCAGCGCGGCGTCGCGCGTCGAGCTCGGCTTGGTTTCCGAGTGTTCGCGCCATGGGA
TTGCAGACAGTGAGGTCGAACAGTTGCTCTTCTCCCTGGGCGTCCAAGTCGTGCCGTTTGATCAACACCATCTCCACTGG
GCCCTCCACGGCTGGCGCCACTACGGCAAGGGTCGCCATCGGGCGGGCCTCAACCTGGGCGATTGCTTCAGTTATGGCCT
GGCCATGGCCCTCGACCTGCCGCTGCTGTTCAAGGGCGACGACTTTGCCCGCACTGAAGTGAAGGTCGCCCTCAGCTGGT
GA

Antitoxin

Nucleotide

• Length: 225 bp

>AT142073 NZ_CP045525:c27978-27754 [Shigella sonnei]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin