Detailed information of TA system TA142072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4505044..4505771
• Replicon: chromosome
• Accession: NZ_CP045524
• Organism: Shigella sonnei strain 6904.27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: GE168_RS22615
• Protein ID: WP_000547563.1
• Coordinates: 4505044..4505355 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GE168_RS22620
• Protein ID: WP_000126296.1
• Coordinates: 4505352..4505771 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE168_RS22585 (4500198)	4500198..4501895	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
GE168_RS22590 (4501905)	4501905..4502447	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
GE168_RS22595 (4502447)	4502447..4503214	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
GE168_RS22600 (4503211)	4503211..4503621	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
GE168_RS22605 (4503614)	4503614..4504084	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
GE168_RS22610 (4504109)	4504109..4504882	+	774	WP_001026459.1	hypothetical protein	-
GE168_RS22615 (4505044)	4505044..4505355	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
GE168_RS22620 (4505352)	4505352..4505771	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
GE168_RS22625 (4505885)	4505885..4507309	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
GE168_RS22630 (4507318)	4507318..4508775	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GE168_RS22635 (4509035)	4509035..4510045	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
GE168_RS22640 (4510193)	4510193..4510720	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T142072 WP_000547563.1 NZ_CP045524:4505044-4505355 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T142072 NZ_CP060395:1473722-1474060 [Synechococcus sp. CBW1108]
GTGGTGAGAGCACCGGCGCGCGGGGCTCTGATCTGGCTCAGCTTCACACCACAGAGTGGGCGTGAACAGGCTGGACGCCG
CCCGGCTTTGGTGGTGTCGTCATCGACGTACAACAGCAAGGTTGGCCTTGCTTTGGTGTGTCCCATCACCAGCAGGGTGA
AGGGATACCCATTTGAAGTGGCGCTGCCGGAAGGGGGGCCGGTGCAGGGGGTGGTGCTGGCGGATCAACTGCGCAGCCTG
GACTGGCGCAGCCGAGAGGCTGAGCTGATTGCCAATGCTCCGATTGCGGTGGTGGAGCGGGTGCTGCAGCTGGTTGGAGC
GCTGTTGAGCAGCCCGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT142072 WP_000126296.1 NZ_CP045524:4505352-4505771 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT142072 NZ_CP060395:1473492-1473728 [Synechococcus sp. CBW1108]
GTGAAAGTGAAGGTGCAGGCCTGGGGCAACAGCCTGGGGCTAAGGATCCCCAAGGCCTATGCCGCCGAGCTGGGTGTGGG
CTCTGGCAGTGAAATGGAGCTGAAGGTGGAGGCCGGTGCCTTGCTCGCCAGGCCCGCAGAGGCGATCCACCTGGATGCCC
TACTGGCTGAGATCACGGCTGAGAACCAACACAGCGAAACTGACTGGGGTGAGCCCACCGGTGCGGAGGTGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22