Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2388371..2388912 Replicon chromosome
Accession NZ_CP045524
Organism Shigella sonnei strain 6904.27

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GE168_RS12215 Protein ID WP_000615976.1
Coordinates 2388634..2388912 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GE168_RS12210 Protein ID WP_000729704.1
Coordinates 2388371..2388631 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GE168_RS12185 (2383840) 2383840..2385552 + 1713 Protein_2386 flagellar biosynthesis protein FlhA -
GE168_RS12190 (2385675) 2385675..2386172 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
GE168_RS12195 (2386243) 2386243..2386371 - 129 Protein_2388 ISAs1 family transposase -
GE168_RS12200 (2386426) 2386426..2387123 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
GE168_RS12205 (2387412) 2387412..2388185 - 774 WP_127148796.1 C40 family peptidase -
GE168_RS12210 (2388371) 2388371..2388631 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GE168_RS12215 (2388634) 2388634..2388912 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GE168_RS12220 (2389068) 2389068..2389808 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
GE168_RS12225 (2389779) 2389779..2390546 - 768 WP_000333380.1 class II glutamine amidotransferase -
GE168_RS12230 (2390752) 2390752..2391330 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2386799..2399773 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T142067 WP_000615976.1 NZ_CP045524:2388634-2388912 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T142067 NZ_CP060395:886587-887027 [Synechococcus sp. CBW1108]
ATGGGCCGGGCCCTGCTCGACATCAACGTGCTGATCGCCCTGCTGGATCAGGGCCATCTGCTGCATCGGCCCGCCACCCG
CTGGCTGGAGGGGCAGCTCGAGCAAGGCTGGGCCACCTGCCCGATCACCGAAAACGGCGTGGTGCGGATCATTGCCCAAC
CGGCTTACCCCAATGCGCAACCGGCGGCCCTGGTGGCCGCACGCCTGGCGGAGGCCTGCAGCCACTCCAGCCATATGTTC
TGGCCGGAGCCGATCAGCCTGCTGCAGGAGGGATTGATCCGCTGGGAGCGGCTGCTGGGCCCGCGCCAGATCACCGATGC
CTACCTGCTGGCCCTGGCCGCCAGCCAGGGCGGCCGCTTCGCCAGCTTCGATCAGCGCATCACTCTTGAAGTGGTGCCCA
GCGCCGAGGCCCACCATCTCTGCCTGATTCCGTGCAGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT142067 WP_000729704.1 NZ_CP045524:2388371-2388631 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT142067 NZ_CP060395:886336-886587 [Synechococcus sp. CBW1108]
ATGCGCACCACGCTCGACATCGAAGACGATGTGCTGGCCGCCGCCCGGGAGCTGGCACGCCGCCAGGGCACCACGGCGGG
CCAGGTGGTGTCGCGCCTGTTGCGAGCTGCGCTCAGCGGCGCACCCTCCACGGGCCAGGCTGCGGCCGAGGCACCACCCA
GCGCGGCTGGATTTCGCCCCTTCCAGGCCCGGGCTGGGGTGGTGGTGACCAATGAGCAGATCGATCAGCTGCGGGATGAG
GCCGGCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References