Detailed information of TA system TA142046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3512467..3513194
• Replicon: chromosome
• Accession: NZ_CP045522
• Organism: Shigella flexneri strain 5908.2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2Y4XNA6
• Locus tag: GE167_RS17835
• Protein ID: WP_000547562.1
• Coordinates: 3512883..3513194 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GE167_RS17830
• Protein ID: WP_000126288.1
• Coordinates: 3512467..3512886 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE167_RS17810 (3507516)	3507516..3508043	-	528	WP_001078777.1	electron transport protein HydN	-
GE167_RS17815 (3508192)	3508192..3509202	-	1011	WP_005051459.1	DNA-binding transcriptional regulator AscG	-
GE167_RS17820 (3509462)	3509462..3510919	+	1458	WP_001107865.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GE167_RS17825 (3510928)	3510928..3512352	+	1425	WP_119182051.1	6-phospho-beta-glucosidase AscB	-
GE167_RS17830 (3512467)	3512467..3512886	-	420	WP_000126288.1	helix-turn-helix domain-containing protein	Antitoxin
GE167_RS17835 (3512883)	3512883..3513194	-	312	WP_000547562.1	type II toxin-antitoxin system HigB family toxin	Toxin
GE167_RS17840 (3513367)	3513367..3514110	-	744	WP_024259998.1	hypothetical protein	-
GE167_RS17845 (3514162)	3514162..3514632	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
GE167_RS17850 (3514625)	3514625..3515035	-	411	WP_001291916.1	formate hydrogenlyase assembly protein HycH	-
GE167_RS17855 (3515032)	3515032..3515799	-	768	WP_025758590.1	formate hydrogenlyase subunit HycG	-
GE167_RS17860 (3515799)	3515799..3516341	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
GE167_RS17865 (3516351)	3516351..3518036	-	1686	WP_025758592.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12298.98 Da
• Isoelectric Point: 9.4924

>T142046 WP_000547562.1 NZ_CP045522:c3513194-3512883 [Shigella flexneri]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T142046 NZ_CP060298:c4385129-4384779 [Nakamurella sp. PAMC28650]
CTGATGCGTCGCGGCGAGATTCGGCTGGTCGATTTGGATCCGGTCAGGGGCAGTGAGGCCAACAAGCAGCGGCCCGCGGT
GGTGGTGAGCAACGATCGGGCTAATTCCGTCGCTCAGAGGCTGGGTCGTGGCGTGGTCACCGTGGTGCCCCTGACCAGTA
ACACCGCGCGGGTGTTTCCGTTCCAGGTGCTGATTCCCGCCGACGCTGTCGGTCTGCGCGTGGCGTCCAAGGCGCAGGCC
GAACAGGTCCGTTCCGTCGCGGTCGAGCGGGTGGGGCCATCTCTGGGTCTGCTGCAGGCATCCTTGTTGGCCGAAATCGA
CGACGCACTTCGTCTCCATCTAGATCTCTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15397.33 Da
• Isoelectric Point: 4.3648

>AT142046 WP_000126288.1 NZ_CP045522:c3512886-3512467 [Shigella flexneri]
MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT142046 NZ_CP060298:c4385344-4385126 [Nakamurella sp. PAMC28650]
ATGAAACTGAGCGTCAGCCTGCCTGACGACGATGTGGCCGTGCTGGATGAGCACGCCCGTAGGGCAGGATTGGGATCTCG
ATCAGCGGCTTTGCACCAGGCCATTCGATTGTTGCGCCGGTCCAGTCTGGAAGACGACTATGCGGCGGCCTGGGAGGACT
GGGACCGGTCAGGTGAGCGTGAGATGTGGGAGACGACGGCATCCGACGGGTTGTCCTGA

Structures

Toxin

• AlphaFold DB: A0A2Y4XNA6