Detailed information of TA system TA142044

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2642909..2643134
• Replicon: chromosome
• Accession: NZ_CP045522
• Organism: Shigella flexneri strain 5908.2

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: GE167_RS13465
• Protein ID: WP_000813254.1
• Coordinates: 2642979..2643134 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2642909..2642967 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE167_RS13425	2638492..2639289	-	798	WP_001325918.1	DgsA anti-repressor MtfA	-
GE167_RS13435	2639500..2640537	-	1038	WP_004974968.1	tyrosine-type recombinase/integrase	-
GE167_RS13440	2640537..2640740	-	204	WP_025759339.1	DUF4224 domain-containing protein	-
GE167_RS13445	2640799..2641113	-	315	WP_001317924.1	3'-5' exonuclease	-
GE167_RS13455	2641911..2642327	+	417	WP_005069274.1	hypothetical protein	-
-	2642909..2642967	-	59	-	-	Antitoxin
GE167_RS13465	2642979..2643134	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
GE167_RS13470	2643302..2643580	+	279	WP_000929754.1	hypothetical protein	-
GE167_RS13475	2643582..2644640	+	1059	WP_025759819.1	DUF968 domain-containing protein	-
GE167_RS13480	2644641..2645006	+	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
GE167_RS13485	2645003..2645718	+	716	Protein_2655	bacteriophage antitermination protein Q	-
GE167_RS13510	2646514..2646729	+	216	WP_000839572.1	class II holin family protein	-
GE167_RS13515	2646828..2647502	+	675	WP_001088287.1	IS66-like element accessory protein TnpA	-
GE167_RS13520	2647499..2647846	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2622617..2687254	64637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T142044 WP_000813254.1 NZ_CP045522:2642979-2643134 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T142044 NZ_CP060298:2065640-2066026 [Nakamurella sp. PAMC28650]
ATGGCTGAGGTAGTCGTCGACGCATCCGCAATGGTCGATCTACTGCTTGACAACGAGTTGGGTAGCGCCGTTCGCCTGCG
ACTCGCGGGACACGCGCTGCACGCGCCGGCCCACCTCGACGCTGAGGTTCTCTCTGCATTAGGACGACTGCATCGGGCTG
GCGACCTCGAGGCCGAAGCCGTCGAATCCAAGCTGCGTGACCTCGTAGCAGCCCCGATCCAGCGGCACGGTGTCGGTGAA
CTTGTCATTGGCGCCTGGTCGCGACGGCATCAGCTACGGCTGGTCGATGCCTTGTACGTCCAGCTGGCCATCGTGCGCGA
TCTTCGGCTGGTCACCACTGACCGCCGTCTTCGGGCCATGCCCTCTGCAGACGTCGTTACCACCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT142044 NZ_CP045522:c2642967-2642909 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin