Detailed information of TA system TA142034

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2249995..2250220
• Replicon: chromosome
• Accession: NZ_CP045522
• Organism: Shigella flexneri strain 5908.2

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: GE167_RS11335
• Protein ID: WP_000813254.1
• Coordinates: 2250065..2250220 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2249995..2250053 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE167_RS24270	2245993..2246162	+	170	Protein_2228	hypothetical protein	-
GE167_RS11300	2246308..2247054	+	747	WP_000788996.1	ATP-binding protein	-
GE167_RS11305	2247069..2247491	+	423	WP_001118171.1	DUF977 family protein	-
GE167_RS11310	2247549..2247905	+	357	WP_000403784.1	hypothetical protein	-
GE167_RS11315	2247998..2248216	+	219	WP_000256997.1	DUF4014 family protein	-
GE167_RS11320	2248218..2248580	+	363	Protein_2233	HNH endonuclease	-
GE167_RS24275	2248580..2249245	+	666	WP_005115317.1	hypothetical protein	-
GE167_RS11330	2249245..2249610	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	2249995..2250053	-	59	-	-	Antitoxin
GE167_RS11335	2250065..2250220	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
GE167_RS11350	2251557..2252156	+	600	WP_000940329.1	DUF1367 family protein	-
GE167_RS11355	2252156..2252446	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
GE167_RS11360	2252443..2252997	+	555	WP_000640143.1	DUF1133 family protein	-
GE167_RS11365	2253409..2254596	-	1188	Protein_2241	IS91 family transposase	-
GE167_RS11370	2254596..2254961	-	366	WP_000124119.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2230689..2258300	27611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T142034 WP_000813254.1 NZ_CP045522:2250065-2250220 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T142034 NZ_CP060289:c4132748-4132566 [Pseudomonas protegens]
ATGCGCAGTCGGGAGATGATCGGCAAGATCGAGGCGGATGGTTGGTACCTGATCGCCGTGAAGGGCAGTCACCATCAGTA
CAAGCATCCCTGGAAGCCAGGGCGCGTCACCATCAGGCACCCGGATGCGGATCTGCCCCGGGGCACGATCAACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT142034 NZ_CP045522:c2250053-2249995 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin