Detailed information of TA system TA142016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 73879..74536
• Replicon: plasmid pM-5360_DHA
• Accession: NZ_CP045510
• Organism: Salmonella enterica subsp. enterica serovar Anatum strain M-5360

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: GE194_RS22705
• Protein ID: WP_000270043.1
• Coordinates: 73879..74229 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GE194_RS22710
• Protein ID: WP_000124640.1
• Coordinates: 74234..74536 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE194_RS22655	69190..69369	+	180	Protein_87	helix-turn-helix transcriptional regulator	-
GE194_RS22660	69434..70138	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
GE194_RS22665	70353..70850	-	498	WP_000062185.1	hypothetical protein	-
GE194_RS22670	70853..71341	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
GE194_RS22675	71438..71773	+	336	WP_000683476.1	hypothetical protein	-
GE194_RS22680	71788..72258	-	471	WP_001281821.1	hypothetical protein	-
GE194_RS22685	72251..72622	-	372	WP_000516916.1	hypothetical protein	-
GE194_RS22690	72633..72827	-	195	WP_000343597.1	hypothetical protein	-
GE194_RS22700	73168..73716	-	549	WP_001061574.1	transcriptional regulator	-
GE194_RS22705	73879..74229	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GE194_RS22710	74234..74536	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
GE194_RS22715	74563..74856	-	294	WP_001239997.1	chromosome segregation protein ParM	-
GE194_RS22720	74944..75216	-	273	WP_001043047.1	HU family DNA-binding protein	-
GE194_RS22725	75274..75801	-	528	WP_001236377.1	thermonuclease family protein	-
GE194_RS22735	76032..76889	-	858	WP_001167032.1	hypothetical protein	-
GE194_RS22740	76876..77106	-	231	WP_000972663.1	hypothetical protein	-
GE194_RS22745	77106..77624	-	519	WP_000210756.1	nitrite reductase	-
GE194_RS22750	77621..78067	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
GE194_RS22755	78067..78426	-	360	WP_000422768.1	hypothetical protein	-
GE194_RS22760	78483..78911	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90966	90966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 15014.38 Da
• Isoelectric Point: 12.8644

>T142016 WP_000270043.1 NZ_CP045510:73879-74229 [Salmonella enterica subsp. enterica serovar Anatum]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 351 bp

>T142016 NZ_CP060281:c5012901-5011984 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 12963.60 Da
• Isoelectric Point: 12.8000

>AT142016 WP_000124640.1 NZ_CP045510:74234-74536 [Salmonella enterica subsp. enterica serovar Anatum]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 303 bp

>AT142016 NZ_CP060281:c5013665-5012907 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9