Detailed information of TA system TA141975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3216447..3217107
• Replicon: chromosome
• Accession: NZ_CP045487
• Organism: Enterobacter cloacae strain 14240244101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9FZF4
• Locus tag: GEA60_RS15325
• Protein ID: WP_014832569.1
• Coordinates: 3216733..3217107 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GEA60_RS15320
• Protein ID: WP_014832568.1
• Coordinates: 3216447..3216749 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA60_RS15300	3213884..3214216	-	333	WP_047360978.1	type II toxin-antitoxin system PemK/MazF family toxin	-
GEA60_RS15305	3214217..3214474	-	258	WP_020687575.1	antitoxin	-
GEA60_RS15310	3214888..3215277	-	390	WP_014832566.1	RidA family protein	-
GEA60_RS15315	3215419..3216351	+	933	WP_203461378.1	LysR family transcriptional regulator	-
GEA60_RS15320	3216447..3216749	-	303	WP_014832568.1	XRE family transcriptional regulator	Antitoxin
GEA60_RS15325	3216733..3217107	-	375	WP_014832569.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GEA60_RS15330	3217293..3217499	-	207	Protein_3006	transcriptional regulator	-
GEA60_RS15335	3217521..3217748	+	228	Protein_3007	glutathione S-transferase	-
GEA60_RS15340	3217798..3218748	-	951	WP_014832572.1	HTH-type transcriptional regulator Cbl	-
GEA60_RS15345	3218845..3219762	-	918	WP_014832573.1	nitrogen assimilation transcriptional regulator NAC	-
GEA60_RS15355	3220253..3221149	-	897	WP_203461379.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14358.33 Da
• Isoelectric Point: 9.7405

>T141975 WP_014832569.1 NZ_CP045487:c3217107-3216733 [Enterobacter cloacae]
VWTINTTDRFDCWFRSLDDTDRASVLAALLVLREKGPALSRPYADTLRGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERTLRDGQKP

Nucleotide

• Length: 375 bp

>T141975 NZ_CP060267:c264974-264639 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGATCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10972.69 Da
• Isoelectric Point: 5.1473

>AT141975 WP_014832568.1 NZ_CP045487:c3216749-3216447 [Enterobacter cloacae]
MGRNLEQLIADEKSDVVASAQAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLTLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT141975 NZ_CP060267:c265251-264964 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAGGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Toxin

• AlphaFold DB: A0A3R9FZF4