Detailed information of TA system TA141944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3387812..3388469
• Replicon: chromosome
• Accession: NZ_CP045485
• Organism: Enterobacter cloacae strain 88701

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GEA56_RS15995
• Protein ID: WP_107533572.1
• Coordinates: 3387812..3388111 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GEA56_RS16000
• Protein ID: WP_107533573.1
• Coordinates: 3388122..3388469 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA56_RS15985	3384117..3386309	+	2193	WP_063451807.1	type I secretion system permease/ATPase	-
GEA56_RS15990	3386329..3387489	+	1161	WP_102751329.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GEA56_RS15995	3387812..3388111	+	300	WP_107533572.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GEA56_RS16000	3388122..3388469	+	348	WP_107533573.1	HigA family addiction module antidote protein	Antitoxin
GEA56_RS16005	3388520..3389701	-	1182	WP_203463657.1	PLP-dependent aminotransferase family protein	-
GEA56_RS16010	3389722..3390609	-	888	WP_102751311.1	LysR family transcriptional regulator	-
GEA56_RS16015	3390707..3391309	+	603	WP_203463658.1	short chain dehydrogenase	-
GEA56_RS16020	3391306..3392037	-	732	WP_203463659.1	class I SAM-dependent methyltransferase	-
GEA56_RS16025	3392112..3393110	-	999	WP_063451815.1	transketolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11524.12 Da
• Isoelectric Point: 10.0104

>T141944 WP_107533572.1 NZ_CP045485:3387812-3388111 [Enterobacter cloacae]
MINHFRDQWLKDFFLYGKSSNVIPSSLESALARKLDMINAATSHRDLRSPPGNMYESLNPPLAGYSAIRVNRQYRLVFRW
SEGKADDLYLSPHKYTPYK

Nucleotide

• Length: 300 bp

>T141944 NZ_CP060243:3955772-3956350 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTTTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13146.07 Da
• Isoelectric Point: 7.0141

>AT141944 WP_107533573.1 NZ_CP045485:3388122-3388469 [Enterobacter cloacae]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAFRLAKAFDTTIEFWLNLQLNVD
VWEAQTNSRTQEELSRIKTVAEIMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT141944 NZ_CP060243:3955129-3955683 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCATCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA