Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 26316..27046 Replicon chromosome
Accession NZ_CP045485
Organism Enterobacter cloacae strain 88701

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag GEA56_RS00135 Protein ID WP_063452067.1
Coordinates 26316..26630 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GEA56_RS00140 Protein ID WP_129741611.1
Coordinates 26627..27046 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GEA56_RS00110 21981..23069 + 1089 WP_063452063.1 cellulase family glycosylhydrolase -
GEA56_RS00115 22972..24156 - 1185 WP_063452213.1 multidrug efflux MFS transporter EmrD -
GEA56_RS00120 24332..25165 - 834 WP_063452064.1 DMT family transporter -
GEA56_RS00125 25228..25674 - 447 WP_063452065.1 GNAT family N-acetyltransferase -
GEA56_RS00130 25739..25828 - 90 WP_045260836.1 type I toxin-antitoxin system toxin TisB -
GEA56_RS00135 26316..26630 + 315 WP_063452067.1 type II toxin-antitoxin system HigB family toxin Toxin
GEA56_RS00140 26627..27046 + 420 WP_129741611.1 helix-turn-helix domain-containing protein Antitoxin
GEA56_RS00145 27193..27291 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
GEA56_RS00150 27398..29086 + 1689 WP_102749937.1 acetolactate synthase large subunit -
GEA56_RS00155 29090..29377 + 288 WP_006808802.1 acetolactate synthase small subunit -
GEA56_RS00160 29449..30042 + 594 WP_203463936.1 transcriptional regulator UhpA -
GEA56_RS00165 30039..31544 + 1506 WP_063452070.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(23-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-137)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12431.53 Da        Isoelectric Point: 10.0527

>T141929 WP_063452067.1 NZ_CP045485:26316-26630 [Enterobacter cloacae]
MHLISMKAICEAVSQFPQYRDELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T141929 NZ_CP060241:1033468-1033746 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATGGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15515.05 Da        Isoelectric Point: 5.5699

>AT141929 WP_129741611.1 NZ_CP045485:26627-27046 [Enterobacter cloacae]
MMIVADALKATHDLVAAVPLLGEHPNEKDYQDALELVEYLLMHEPGSPLLDIVCARISRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQHKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT141929 NZ_CP060241:1033758-1034063 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References