Detailed information of TA system TA141765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4435..5092
• Replicon: plasmid pTHAF12_a
• Accession: NZ_CP045400
• Organism: Halomonas sp. THAF12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FIU88_RS17740
• Protein ID: WP_152480276.1
• Coordinates: 4748..5092 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FIU88_RS17735
• Protein ID: WP_152480275.1
• Coordinates: 4435..4737 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU88_RS17725	2568..3602	-	1035	WP_152480273.1	plasmid replication protein	-
FIU88_RS17730	3639..3998	-	360	WP_152480274.1	H-NS histone family protein	-
FIU88_RS17735	4435..4737	-	303	WP_152480275.1	helix-turn-helix domain-containing protein	Antitoxin
FIU88_RS17740	4748..5092	-	345	WP_152480276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU88_RS17745	5315..5587	-	273	WP_152480277.1	hypothetical protein	-
FIU88_RS17750	5584..6525	-	942	WP_152480278.1	integrase domain-containing protein	-
FIU88_RS17755	8211..9143	+	933	WP_172977769.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..169237	169237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13223.91 Da
• Isoelectric Point: 4.7453

>T141765 WP_152480276.1 NZ_CP045400:c5092-4748 [Halomonas sp. THAF12]
MWSIEQTETFEDWYYSLDDVDRENVLASMLLLQEKGPMLSRPHADTVNGSRYPNMKELRIQSQGKPFRAFFAFDPNRTGL
LLCAGDKGGNEKRFYDEMIPIADREYAAHLESLK

Nucleotide

• Length: 345 bp

>T141765 NZ_CP060083:3016319-3016426 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10995.77 Da
• Isoelectric Point: 7.2056

>AT141765 WP_152480275.1 NZ_CP045400:c4737-4435 [Halomonas sp. THAF12]
MARKLEQLLANEKPEVVAAAKAKADEMLLELRLSEVRRLVEMTQAQMADAMGVRQPTVAGMEKGGQDLRLSSIKRYVEAV
GGRVRLDVELPDGSHHGFPV

Nucleotide

• Length: 303 bp

>AT141765 NZ_CP060083:c3016272-3016206 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT