Detailed information of TA system TA141761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 16249..16763
• Replicon: plasmid pTHAF27_c
• Accession: NZ_CP045396
• Organism: Roseovarius sp. THAF27

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: FIU89_RS22000
• Protein ID: WP_152494729.1
• Coordinates: 16249..16515 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: FIU89_RS22005
• Protein ID: WP_036743750.1
• Coordinates: 16512..16763 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU89_RS21975	11421..13094	+	1674	WP_152494724.1	type IV secretory system conjugative DNA transfer family protein	-
FIU89_RS21980	13113..13661	+	549	WP_152494725.1	hypothetical protein	-
FIU89_RS21985	14000..15064	+	1065	WP_152494726.1	replication initiator protein A	-
FIU89_RS21990	15061..15375	-	315	WP_152494727.1	hypothetical protein	-
FIU89_RS21995	15372..16100	-	729	WP_152494728.1	ParA family protein	-
FIU89_RS22000	16249..16515	-	267	WP_152494729.1	Txe/YoeB family addiction module toxin	Toxin
FIU89_RS22005	16512..16763	-	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FIU89_RS22010	16870..17556	-	687	WP_152494730.1	helix-turn-helix transcriptional regulator	-
FIU89_RS22015	17811..18440	+	630	WP_172978219.1	tyrosine-type recombinase/integrase	-
FIU89_RS22020	18440..18691	+	252	WP_152494732.1	WGR domain-containing protein	-
FIU89_RS22025	18856..19119	+	264	WP_152494733.1	BrnT family toxin	-
FIU89_RS22030	19106..19321	+	216	WP_152494734.1	CopG family transcriptional regulator	-
FIU89_RS22035	19465..20013	+	549	Protein_23	IS5 family transposase	-
FIU89_RS22040	19997..20854	-	858	Protein_24	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..23734	23734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10393.75 Da
• Isoelectric Point: 9.7931

>T141761 WP_152494729.1 NZ_CP045396:c16515-16249 [Roseovarius sp. THAF27]
VKLVFSDQAWEDYQYWVNTNDKVRDRINDLIKQCKRTPFKGTGKPEPLKGDLTGWWSRRISKEDRMVYRVSGAGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T141761 NZ_CP060083:c2533239-2532910 [Escherichia coli]
ATGAACTACATTATCGAATACTACAGTGACGAGGTTGAAGCTGAGATCCTTTCTCTTCCTGAGACCTTACAAGCCCGGTA
CATTCGGTACACGGAGAAAATGCGTATTTACGGAGCTAATCTTGGTTCCCCGCATACAGAGGCATTTGGCGACGGTTTGT
TTGAAATCCGCCTTAAGGGCTCGGAAGGCATAGGGCGTGTTTTTTACTGCACGCTAAAAGGAAAGCGAATCATTATGTTG
CATAGCTTTGTAAAGAAAACGCAAAAAACTCCACCTGCCGAGCTTAGAAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT141761 WP_036743750.1 NZ_CP045396:c16763-16512 [Roseovarius sp. THAF27]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT141761 NZ_CP060083:c2532920-2532612 [Escherichia coli]
ATGACTGGTAAACGTACCCCACCTACCATGACACACGATGATATGGTAGAAAAAATGTTGTCTAACCCCGCAGTAAAAGC
AGAATACGACGCCATCGCCGATGAATTCGCACTACTTGATGAAATGCTGGCAGCACGAAAAGAAGCTGGCTTAACCCAGG
CTGAAGTTGCTGAACGTATGGGAACAAAAGCGACTGCGATAACCAGAATGGAAAGCAATCTTGCATCAGGTACAAGCGGC
CCATCATTTGCCACACTGAAAAAATTCGCCCGTGCTACAGGGAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5