Detailed information of TA system TA141730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3067056..3067705
• Replicon: chromosome
• Accession: NZ_CP045349
• Organism: Pseudomonas sp. THAF187a

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FIV02_RS14285
• Protein ID: WP_152488990.1
• Coordinates: 3067056..3067400 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1H0F3K8
• Locus tag: FIV02_RS14290
• Protein ID: WP_074879837.1
• Coordinates: 3067397..3067705 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIV02_RS14270	3062815..3064668	+	1854	WP_152488987.1	asparagine synthase (glutamine-hydrolyzing)	-
FIV02_RS14275	3064753..3065952	+	1200	WP_152488988.1	MFS transporter	-
FIV02_RS14280	3065966..3066892	+	927	WP_152488989.1	LysR family transcriptional regulator	-
FIV02_RS14285	3067056..3067400	+	345	WP_152488990.1	toxin	Toxin
FIV02_RS14290	3067397..3067705	+	309	WP_074879837.1	transcriptional regulator	Antitoxin
FIV02_RS14295	3068133..3068900	-	768	WP_152488991.1	hypothetical protein	-
FIV02_RS14300	3068897..3071359	-	2463	WP_152488992.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2975614..3093254	117640
	-	flank	IS/Tn	-	-	3071758..3073188	1430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13303.14 Da
• Isoelectric Point: 9.6940

>T141730 WP_152488990.1 NZ_CP045349:3067056-3067400 [Pseudomonas sp. THAF187a]
MKALFVELSPFERNRKAHLDDDEYSLFQQMLLANPEAGAVIANSGGLRKVRFGSVRRNKGKRGGVRVIYYYWHSGLQFWL
FTLYDKDELDDLSSDQRRQLKALLEHEVKARETP

Nucleotide

• Length: 345 bp

>T141730 NZ_CP060075:c648276-647812 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGAGCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11575.36 Da
• Isoelectric Point: 10.0927

>AT141730 WP_074879837.1 NZ_CP045349:3067397-3067705 [Pseudomonas sp. THAF187a]
MTKRDIFSELIEGFDDLAAAREGKRTLRTHKVEYKPIEPATGAEIVALREKLQMSQGVFAQAIHAKPATLKNWEQNRSKP
NDQATVLIRILSKHPELIRELA

Nucleotide

• Length: 309 bp

>AT141730 NZ_CP060075:c648611-648276 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1H0F3K8