Detailed information of TA system TA141683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 83006..83700
• Replicon: plasmid unnamed1
• Accession: NZ_CP045303
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GCU53_RS24070
• Protein ID: WP_152390103.1
• Coordinates: 83410..83700 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GCU53_RS24065
• Protein ID: WP_152390102.1
• Coordinates: 83006..83407 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS24020	78313..78735	-	423	WP_152390095.1	type II toxin-antitoxin system VapC family toxin	-
GCU53_RS24025	78735..78986	-	252	WP_152390096.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GCU53_RS24035	79671..79970	+	300	WP_152390097.1	hypothetical protein	-
GCU53_RS24040	79918..80352	-	435	WP_152390098.1	integrase core domain-containing protein	-
GCU53_RS24045	80729..80992	+	264	WP_152390099.1	hypothetical protein	-
GCU53_RS24050	81071..81676	+	606	WP_152390100.1	3'-5' exonuclease	-
GCU53_RS24055	82225..82515	-	291	WP_152390101.1	HU family DNA-binding protein	-
GCU53_RS24060	82400..82969	+	570	Protein_107	transposase	-
GCU53_RS24065	83006..83407	-	402	WP_152390102.1	helix-turn-helix domain-containing protein	Antitoxin
GCU53_RS24070	83410..83700	-	291	WP_152390103.1	type II toxin-antitoxin system HigB family toxin	Toxin
GCU53_RS24075	84086..85036	+	951	WP_152390104.1	replication terminus site-binding protein	-
GCU53_RS24080	85164..86029	+	866	Protein_111	transposase	-
GCU53_RS24085	86063..87037	-	975	WP_208845544.1	ParB/RepB/Spo0J family partition protein	-
GCU53_RS24090	87172..88296	-	1125	WP_152390105.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..321600	321600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11311.10 Da
• Isoelectric Point: 10.3557

>T141683 WP_152390103.1 NZ_CP045303:c83700-83410 [Azotobacter salinestris]
MHVITQKRIWEAKAKWPHAATALEAWYRLIKASAPQDFAEMKRLFPATDKVGPQHVFDIGGNKLRLIAVVQYRAQKVYIQ
HVLDHVEYDNRSKWRE

Nucleotide

• Length: 291 bp

>T141683 NZ_CP060061:c4083887-4083489 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14795.77 Da
• Isoelectric Point: 4.4675

>AT141683 WP_152390102.1 NZ_CP045303:c83407-83006 [Azotobacter salinestris]
MSALIKQASEHWRYVAPLLTRPANEDDYDALVEALDELLVIVGDDEDHPLATLASHMGDLIEAYDEAHHPMPKVSGVEML
RYLMQEHGLTQADLPEVGAQSVVSELLSGKRQLNVRHIRALSERFGVPADVFL

Nucleotide

• Length: 402 bp

>AT141683 NZ_CP060061:c4084183-4083890 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA