Detailed information of TA system TA141670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 860251..860813
• Replicon: chromosome
• Accession: NZ_CP045302
• Organism: Azotobacter salinestris strain KACC 13899

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GCU53_RS04030
• Protein ID: WP_152386476.1
• Coordinates: 860544..860813 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GCU53_RS04025
• Protein ID: WP_152386475.1
• Coordinates: 860251..860538 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCU53_RS03985	855844..856155	-	312	WP_152386471.1	hypothetical protein	-
GCU53_RS03990	856397..856813	-	417	WP_152386472.1	hypothetical protein	-
GCU53_RS03995	856886..857344	-	459	WP_152386473.1	Hsp20 family protein	-
GCU53_RS04000	858156..858545	-	390	WP_208845439.1	hypothetical protein	-
GCU53_RS04005	859109..859384	-	276	WP_152386474.1	helix-turn-helix domain-containing protein	-
GCU53_RS04010	859455..859782	-	328	Protein_735	type II toxin-antitoxin system HicB family antitoxin	-
GCU53_RS04015	859809..859892	-	84	Protein_736	transcriptional regulator	-
GCU53_RS04020	859925..860239	-	315	Protein_737	helix-turn-helix domain-containing protein	-
GCU53_RS04025	860251..860538	-	288	WP_152386475.1	helix-turn-helix domain-containing protein	Antitoxin
GCU53_RS04030	860544..860813	-	270	WP_152386476.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCU53_RS04035	861241..861636	+	396	WP_152386477.1	hypothetical protein	-
GCU53_RS04040	861856..862245	-	390	WP_152386478.1	hypothetical protein	-
GCU53_RS04045	862541..862984	-	444	WP_152386479.1	holin family protein	-
GCU53_RS04050	863142..863378	-	237	WP_152386480.1	hypothetical protein	-
GCU53_RS04055	863489..864376	-	888	WP_152389777.1	EamA family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (28-84)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 9997.34 Da
• Isoelectric Point: 6.4629

>T141670 WP_152386476.1 NZ_CP045302:c860813-860544 [Azotobacter salinestris]
MLDDDSYAAFQKALAERPDVGDIIEGTGGIRKVRVASKGHGKRDGSQAIYYHFVSESQIALLLIYPKNEKDDLTADERKA
LKCIIETWR

Nucleotide

• Length: 270 bp

>T141670 NZ_CP060061:c785899-785525 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10715.25 Da
• Isoelectric Point: 7.2057

>AT141670 WP_152386475.1 NZ_CP045302:c860538-860251 [Azotobacter salinestris]
MSKFSDDLMESVVQMDEIVRGERQPSREFTVDALQVKQIRKATGLSQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPEHVIRALAD

Nucleotide

• Length: 288 bp

>AT141670 NZ_CP060061:c786357-785989 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA