Detailed information of TA system TA141647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 335236..335859
• Replicon: chromosome
• Accession: NZ_CP045291
• Organism: Delftia tsuruhatensis strain TR1180

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6AKU7
• Locus tag: GCS91_RS01510
• Protein ID: WP_013800140.1
• Coordinates: 335236..335517 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GCS91_RS01515
• Protein ID: WP_034399070.1
• Coordinates: 335560..335859 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCS91_RS01485	330468..331331	+	864	WP_016448674.1	branched-chain amino acid ABC transporter permease	-
GCS91_RS01490	331332..332306	+	975	WP_046238969.1	branched-chain amino acid ABC transporter permease	-
GCS91_RS01495	332293..333057	+	765	WP_016452146.1	ABC transporter ATP-binding protein	-
GCS91_RS01500	333044..333751	+	708	WP_013800138.1	ABC transporter ATP-binding protein	-
GCS91_RS01505	333930..335106	+	1177	Protein_299	ABC transporter substrate-binding protein	-
GCS91_RS01510	335236..335517	+	282	WP_013800140.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCS91_RS01515	335560..335859	+	300	WP_034399070.1	HigA family addiction module antidote protein	Antitoxin
GCS91_RS01520	335952..336725	-	774	WP_154833118.1	SDR family oxidoreductase	-
GCS91_RS01525	336859..337665	-	807	WP_016448668.1	IclR family transcriptional regulator	-
GCS91_RS01530	337710..338483	-	774	WP_016448667.1	ferredoxin--NADP reductase	-
GCS91_RS01535	338745..339680	-	936	WP_154833120.1	LysR family transcriptional regulator	-
GCS91_RS01540	339774..340565	+	792	WP_154833122.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11067.70 Da
• Isoelectric Point: 9.3577

>T141647 WP_013800140.1 NZ_CP045291:335236-335517 [Delftia tsuruhatensis]
MTIQSFKCPDTEQLFQQRRVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALHGDRKGQHSIRINDQWRVCFVWT
HEGPSELEIVDYH

Nucleotide

• Length: 282 bp

>T141647 NZ_CP060057:928896-929303 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11023.66 Da
• Isoelectric Point: 5.0190

>AT141647 WP_034399070.1 NZ_CP045291:335560-335859 [Delftia tsuruhatensis]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIVKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADM
ARKSIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT141647 NZ_CP060057:928649-928915 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A5P8XEH0