Detailed information of TA system TA141646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 19687..20333
• Replicon: plasmid pLAU-OXA4
• Accession: NZ_CP045281
• Organism: Escherichia coli strain LAU-OXA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E9TBD5
• Locus tag: GBF15_RS24985
• Protein ID: WP_000269927.1
• Coordinates: 19983..20333 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: E9TBD4
• Locus tag: GBF15_RS24980
• Protein ID: WP_001259442.1
• Coordinates: 19687..19986 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBF15_RS24940	15061..15369	+	309	WP_131199878.1	CcgAII protein	-
GBF15_RS24945	15435..16085	+	651	WP_001330913.1	hypothetical protein	-
GBF15_RS24950	16296..16781	+	486	WP_131199877.1	hypothetical protein	-
GBF15_RS24955	17354..17578	+	225	WP_050418674.1	hypothetical protein	-
GBF15_RS24960	17611..17961	+	351	WP_000694584.1	hypothetical protein	-
GBF15_RS24965	18281..18904	+	624	WP_001127156.1	ParA family protein	-
GBF15_RS24970	18901..19110	+	210	WP_000041404.1	hypothetical protein	-
GBF15_RS24975	19138..19629	+	492	WP_000456858.1	hypothetical protein	-
GBF15_RS24980	19687..19986	-	300	WP_001259442.1	XRE family transcriptional regulator	Antitoxin
GBF15_RS24985	19983..20333	-	351	WP_000269927.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBF15_RS24995	20691..21194	+	504	WP_021526598.1	hypothetical protein	-
GBF15_RS25000	21363..21731	+	369	WP_024186959.1	hypothetical protein	-
GBF15_RS25010	22299..22895	-	597	WP_021528497.1	hypothetical protein	-
GBF15_RS25015	22892..23419	-	528	WP_024186961.1	hypothetical protein	-
GBF15_RS25020	23431..23646	+	216	WP_001280801.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..29825	29825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13770.78 Da
• Isoelectric Point: 7.4219

>T141646 WP_000269927.1 NZ_CP045281:c20333-19983 [Escherichia coli]
MWTVYFGRLFDEWFEEQELALKRKVLAELRHLEEFGPSLSRPHADTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAI
ILCAGDKSNDKKFYDRMIRIADEEFSTHLAELEGKK

Nucleotide

• Length: 351 bp

>T141646 NZ_CP060057:c785057-784683 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10931.73 Da
• Isoelectric Point: 4.6283

>AT141646 WP_001259442.1 NZ_CP045281:c19986-19687 [Escherichia coli]
MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT141646 NZ_CP060057:c785515-785147 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: E9TBD5

Antitoxin

• AlphaFold DB: E9TBD4