Detailed information of TA system TA141639

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40154..40580
• Replicon: plasmid pLAU-OXA2
• Accession: NZ_CP045279
• Organism: Escherichia coli strain LAU-OXA

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GBF15_RS24365
• Protein ID: WP_001312861.1
• Coordinates: 40154..40312 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40356..40580 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBF15_RS24320	35359..35493	-	135	Protein_36	TraY domain-containing protein	-
GBF15_RS24325	35581..36258	-	678	WP_001348626.1	PAS domain-containing protein	-
GBF15_RS24330	36392..36775	-	384	WP_001151566.1	relaxosome protein TraM	-
GBF15_RS24335	37106..37708	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
GBF15_RS24340	38005..38826	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GBF15_RS24345	38945..39232	-	288	WP_000107535.1	hypothetical protein	-
GBF15_RS24350	39257..39463	-	207	WP_000275859.1	hypothetical protein	-
GBF15_RS24365	40154..40312	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	40356..40580	-	225	NuclAT_0	-	Antitoxin
-	40356..40580	-	225	NuclAT_0	-	Antitoxin
-	40356..40580	-	225	NuclAT_0	-	Antitoxin
-	40356..40580	-	225	NuclAT_0	-	Antitoxin
GBF15_RS25610	40392..40580	+	189	WP_001299721.1	hypothetical protein	-
GBF15_RS24370	40592..41311	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
GBF15_RS24375	41308..41742	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
GBF15_RS24380	41797..43755	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
GBF15_RS24385	43821..44054	-	234	WP_000005990.1	DUF905 family protein	-
GBF15_RS24390	44117..44614	-	498	WP_042045560.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82182	82182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T141639 WP_001312861.1 NZ_CP045279:c40312-40154 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T141639 NZ_CP060051:24947-25252 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT141639 NZ_CP045279:c40580-40356 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin