Detailed information of TA system TA141635

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 17927..18166
• Replicon: plasmid pLAU-OXA2
• Accession: NZ_CP045279
• Organism: Escherichia coli strain LAU-OXA

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: GBF15_RS24250
• Protein ID: WP_023144756.1
• Coordinates: 17927..18061 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	18106..18166 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBF15_RS24210	13274..13689	-	416	Protein_15	IS1 family transposase	-
GBF15_RS24215	13938..14339	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
GBF15_RS24220	14272..14529	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
GBF15_RS24225	14622..15275	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
GBF15_RS24235	16215..17072	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
GBF15_RS24240	17065..17139	-	75	WP_031943482.1	RepA leader peptide Tap	-
GBF15_RS25605	17136..17270	-	135	Protein_21	protein CopA/IncA	-
GBF15_RS24245	17376..17630	-	255	WP_000083850.1	replication regulatory protein RepA	-
GBF15_RS24250	17927..18061	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	18106..18166	+	61	NuclAT_1	-	Antitoxin
-	18106..18166	+	61	NuclAT_1	-	Antitoxin
-	18106..18166	+	61	NuclAT_1	-	Antitoxin
-	18106..18166	+	61	NuclAT_1	-	Antitoxin
GBF15_RS24255	18133..18419	-	287	Protein_24	DUF2726 domain-containing protein	-
GBF15_RS24265	18932..19144	-	213	WP_013023861.1	hypothetical protein	-
GBF15_RS24270	19275..19835	-	561	WP_000139341.1	fertility inhibition protein FinO	-
GBF15_RS24275	19890..20636	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82182	82182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T141635 WP_023144756.1 NZ_CP045279:c18061-17927 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T141635 NZ_CP060049:c4775030-4774746 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT141635 NZ_CP045279:18106-18166 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin