Detailed information of TA system TA141627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3758885..3759579
• Replicon: chromosome
• Accession: NZ_CP045277
• Organism: Escherichia coli strain LAU-OXA

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: GBF15_RS18385
• Protein ID: WP_001263489.1
• Coordinates: 3758885..3759283 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: GBF15_RS18390
• Protein ID: WP_000554758.1
• Coordinates: 3759286..3759579 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3754473..3754553	-	81	NuclAT_12	-	-
-	3754473..3754553	-	81	NuclAT_12	-	-
-	3754473..3754553	-	81	NuclAT_12	-	-
-	3754473..3754553	-	81	NuclAT_12	-	-
GBF15_RS18360	3755149..3755607	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GBF15_RS18365	3755868..3757325	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
GBF15_RS18370	3757382..3757903	-	522	Protein_3545	peptide chain release factor H	-
GBF15_RS18375	3757899..3758105	-	207	Protein_3546	RtcB family protein	-
GBF15_RS18380	3758423..3758875	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
GBF15_RS18385	3758885..3759283	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GBF15_RS18390	3759286..3759579	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GBF15_RS18395	3759631..3760686	-	1056	WP_001226164.1	DNA polymerase IV	-
GBF15_RS18400	3760757..3761542	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
GBF15_RS18405	3761487..3763226	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
GBF15_RS18410	3763450..3763947	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3710475..3764749	54274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T141627 WP_001263489.1 NZ_CP045277:c3759283-3758885 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T141627 NZ_CP060049:730890-731375 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT141627 WP_000554758.1 NZ_CP045277:c3759579-3759286 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT141627 NZ_CP060049:730600-730893 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3