Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1824417..1824919 Replicon chromosome
Accession NZ_CP045277
Organism Escherichia coli strain LAU-OXA

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GBF15_RS08665 Protein ID WP_000767829.1
Coordinates 1824665..1824919 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GBF15_RS08660 Protein ID WP_001259255.1
Coordinates 1824417..1824668 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBF15_RS08630 1819595..1820662 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GBF15_RS08635 1820662..1821732 - 1071 WP_001518029.1 histidinol-phosphate transaminase -
GBF15_RS08640 1821729..1823033 - 1305 WP_001367667.1 histidinol dehydrogenase -
GBF15_RS08645 1823039..1823938 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
GBF15_RS08650 1824084..1824134 - 51 WP_001364200.1 his operon leader peptide -
GBF15_RS08660 1824417..1824668 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GBF15_RS08665 1824665..1824919 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GBF15_RS08670 1825002..1825826 + 825 WP_000754737.1 SDR family oxidoreductase -
GBF15_RS08675 1825872..1826801 + 930 WP_000803351.1 LysR family transcriptional regulator -
GBF15_RS08680 1827016..1827078 + 63 WP_010723108.1 membrane protein YoeI -
GBF15_RS08685 1827068..1828426 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GBF15_RS08690 1828605..1829663 + 1059 WP_000492333.1 transport protein YeeE -
GBF15_RS08695 1829677..1829904 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T141614 WP_000767829.1 NZ_CP045277:1824665-1824919 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T141614 NZ_CP060042:c147279-146986 [Burkholderia cenocepacia]
ATGAGAATCGAGCAGACAGACGACTTCGCGAAATGGCTGCGCGGCCTACGTGACCACATCGCGCGCGCGCAGATCGCCAA
GCGGATTCAACGGCTCGCGCGCGGCCAGTACGGGGACGTGAAGTCGGTTGGTGCCGGGGTGAGCGAAATGCGCGTGCACG
TCGGCCCCGGCTATCGCGTCTACTTTGTCCAACGAGGCTCAACGCTCGTCATCCTGCTATGCGGTGGCGACAAGTCAACG
CAGCAGCGCGACATTGAGCGCGCTATCGCGCTTTCGGCGCAATTGGGAGATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT141614 WP_001259255.1 NZ_CP045277:1824417-1824668 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT141614 NZ_CP060042:c146984-146685 [Burkholderia cenocepacia]
ATGGGTATCAAGACAACGCCGTTTGACGCGGCCGAATACCTCGACACGCCCGAGGCGCAGGCCGAATATCTGCGGGTTTC
GTTCGAGACTGGCGATTCTGCCGACATTCAGGAAGCCATCGGCACTGTGGCGCGTGCGCGCGGCATGACGCAGATTGCGC
GCGACGCGGGCGTTGGCCGCGAAAGCCTCTACAAAGCGCTGGACGAGCACGGCAACCCGGAATTCGCGACGATCCTGCGT
GTGGTGCGCGCGCTCGGCCTCACTTTGACCGTAACGACGCCTGTACACGAGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References