Detailed information of TA system TA141605

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128156..128409
• Replicon: plasmid p16HN-263_KPC
• Accession: NZ_CP045264
• Organism: Klebsiella pneumoniae strain 16HN-263

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: ELO34_RS29230
• Protein ID: WP_001312851.1
• Coordinates: 128260..128409 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	128156..128215 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELO34_RS29190	123816..123881	-	66	Protein_164	helix-turn-helix domain-containing protein	-
ELO34_RS29195	123934..124638	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
ELO34_RS29200	124702..124863	+	162	Protein_166	DNA helicase	-
ELO34_RS29205	124883..125629	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
ELO34_RS29210	125684..126244	+	561	WP_000139328.1	fertility inhibition protein FinO	-
ELO34_RS29215	126376..126576	+	201	WP_015059022.1	hypothetical protein	-
ELO34_RS29220	126962..127561	+	600	WP_032083981.1	hypothetical protein	-
ELO34_RS29225	127725..127955	+	231	WP_001736714.1	hypothetical protein	-
-	128156..128215	-	60	NuclAT_1	-	Antitoxin
-	128156..128215	-	60	NuclAT_1	-	Antitoxin
-	128156..128215	-	60	NuclAT_1	-	Antitoxin
-	128156..128215	-	60	NuclAT_1	-	Antitoxin
ELO34_RS29230	128260..128409	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
ELO34_RS29235	128693..128941	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 / catA2	-	1..129252	129252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T141605 WP_001312851.1 NZ_CP045264:128260-128409 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T141605 NZ_CP060040:c1355145-1354726 [Burkholderia cenocepacia]
ATGATCCTCGTCGATACCAACGTCATTTCCGAGCCGCTGCGGCGCGAGCCGAACGCGGCCGTGATCGAATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTTCTTGCCGCGATCAGTCTGGCGGAAATGCGCTTCGGCGTGGCGGCGTTGCCGGAGGGCC
GTCGGCGGGACTGGCTCGAGCAAAGTATCGAGCAGCGCGTCGTGCCGTTGTTTCGCGGCCGGGTCCTGCCGTTCGATGAT
GCGGCCAGCAAGGCGTACGCGAGCCTGCGCGCCAAGGCGCGTGGTACGGGCAACGCGATGGCGTCTGCCGACGGCTTCAT
TGCCGCAACCGCCGCGGCGAACGGGCTGATCGTGGCCACGCGCGACGTCGCGCCGTTCGAGGCGGCAGGCCTGCGTGTGA
TCGACCCTTGGGCCGCCTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT141605 NZ_CP045264:c128215-128156 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin