Detailed information of TA system TA141601

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37150..37419
• Replicon: plasmid p16HN-263_KPC
• Accession: NZ_CP045264
• Organism: Klebsiella pneumoniae strain 16HN-263

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ELO34_RS28510
• Protein ID: WP_001312861.1
• Coordinates: 37261..37419 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37150..37215 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELO34_RS28485	32860..33387	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
ELO34_RS28490	33445..33678	+	234	WP_000006003.1	DUF905 family protein	-
ELO34_RS28495	33739..35762	+	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
ELO34_RS28500	35831..36265	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
ELO34_RS28505	36262..36981	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36993..37217	+	225	NuclAT_0	-	-
-	36993..37217	+	225	NuclAT_0	-	-
-	36993..37217	+	225	NuclAT_0	-	-
-	36993..37217	+	225	NuclAT_0	-	-
-	37150..37215	+	66	-	-	Antitoxin
ELO34_RS28510	37261..37419	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ELO34_RS29560	37657..38034	-	378	Protein_49	hypothetical protein	-
ELO34_RS28525	38334..38630	+	297	WP_001272251.1	hypothetical protein	-
ELO34_RS28530	38741..39562	+	822	WP_001234445.1	DUF945 domain-containing protein	-
ELO34_RS28535	39859..40506	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
ELO34_RS28540	40783..41166	+	384	WP_000124981.1	relaxosome protein TraM	-
ELO34_RS28545	41357..42043	+	687	WP_015059009.1	PAS domain-containing protein	-
ELO34_RS28550	42137..42364	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 / catA2	-	1..129252	129252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T141601 WP_001312861.1 NZ_CP045264:37261-37419 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T141601 NZ_CP060040:887451-887744 [Burkholderia cenocepacia]
ATGTTCAAAGTCTTGACCCCCCAATTTGATAGCTGGCTCGACGGGCTTCGCGATCCAGTCGGCAGCGCGGCAATCAGCCT
GCGCATCGAGCGGGCGAAGCTCGGCAACCTCGGCCAGTGGCGCGCAGTCGGTGACGGCGTCAATGAAATGAAGATCGACG
TAGGGCCGGGATACCGGGCCTACTTCGTGCGGCGCGGAAAAATCATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCGACG
CAGAAGAAGGACATCAAGACGGCGAAGCAAATCGCCGGCGAACTGGAGGATTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT141601 NZ_CP045264:37150-37215 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin